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Glycoconjugate Journal

, Volume 9, Issue 1, pp 21–26 | Cite as

Detection of the gangliosideN-glycolyl-neuraminyl-lactosyl-ceramide by biotinylatedEscherichia coli K99 lectin

  • Abdelouahab Ouadia
  • Yannis Karamanos
  • Raymond Julien
Papers

Abstract

K99 lectin fromEscherichia coli was purified and biotinylated via its carboxyl groups using biocytin hydrazide and a water soluble carbodiimide. Biotinylation of two out of the nine carboxyl groups was sufficient to permit detection of the lectin by avidin and did not cause any loss of the haemagglutinating activity. It was demonstrated that the biotinylated K99 lectin retained other important properties of native K99 and that it will probably become a very sensitive detecting reagent. Indeed, it was able to bind to HeLa cells, as do intact bacteria carrying K99 fimbriae, and also to recognizeN-glycolyl-neuraminyl-lactosyl-ceramide in an overlay binding assay.

Keywords

K99 lectin NeuGe-GM3 detection 

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Copyright information

© Chapman and Hall 1992

Authors and Affiliations

  • Abdelouahab Ouadia
    • 1
  • Yannis Karamanos
    • 1
  • Raymond Julien
    • 1
  1. 1.Institut de BiotechnologieUniversité de LimogesLimoges cedexFrance

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