Glycoconjugate Journal

, Volume 11, Issue 2, pp 105–110 | Cite as

Enzymic synthesis of the trisaccharide core region of the carbohydrate chain ofN-glycoprotein

  • Taichi Usui
  • Masahiro Suzuki
  • Toshinari Sato
  • Hirokazu Kawagishi
  • Kyoko Adachi
  • Hiroshi Sano
Glycopinion Mini-Review


Transmannosylation from mannotriose (Manβ1-4Manβ1-4Man) to the 4-position at the nonreducing end N-acetylglucosaminyl residue ofN,N′-diacetylchitobiose was regioselectively induced through the use of β-d-mannanase fromAspergillus niger. The enzyme formed the trisaccharide Manβ1-4GlcNAcβ1-4GlcNAc (3.7% of the enzyme-catalysed net decrease ofN,N′-diacetylchitobiose) from mannotriose as a donor andN,N′-diacetylchitobiose as an acceptor. Mannobiose (Manβ1-4Man) was also shown to be useful as a donor substrate for the desired trisaccharide synthesis.


Manβ1-4GlcNAcβ1-4GlcNAc β-d-mannanase in carbohydrate synthesis trisaccharide core ofN-glycoprotein 




(Mn) (n=1−5)

β-linkedn-mer of mannose




Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Sukeno T, Tarentino AI, Plummer TH, Maley E (1972)Biochemistry 11:1493–1501.Google Scholar
  2. 2.
    Sugahara K, Okumura T, Yamashina I (1972)Biochim Biophys Acta 268:488–496.Google Scholar
  3. 3.
    Goldstein IJ, Hayes CE (1978)Carbohydr Chem Biochem 35:127–340.Google Scholar
  4. 4.
    Waechter CJ, Lennaz W-J (1976)Annu Rev Biochem 41:95–112.Google Scholar
  5. 5.
    Warren CD, Auge C, Larer ML, Suzuki S, Power D, Jeanloz RW (1980)Carbohydr Res 82:71–83.Google Scholar
  6. 6.
    Auge C, Warren CD, Jeanloz RW (1980)Carbohydr Res 82:85–95.Google Scholar
  7. 7.
    Ogawa T, Kitajima T, Nukada T (1983)Carbohydr Res 123:C5-C7.Google Scholar
  8. 8.
    Paulsen H, Lebuhn R (1984)Carbohydr Res 130:85–101.Google Scholar
  9. 9.
    Wallenfeld K, Well R (1972)The Enzymes 3:617–663. Academic Press, New York.Google Scholar
  10. 10.
    Hedbys L, Larsson PO, Mosbach K, Svenson (1984)Biochim Biophys Res. Commun 123:8–15.Google Scholar
  11. 11.
    Kusakabe I, Takahashi R, Murakami K, Maekawa, Suzuki T (1983)Agric Biol Chem 47:2391–2392.Google Scholar
  12. 12.
    Somogyi M (1952)J Biol Chem 195:19–23.Google Scholar
  13. 13.
    Nelson N (1944)J Biol Chem 53:375–380.Google Scholar
  14. 14.
    Ogura K, Tsurugi J, Watanabe T (1973)Carbohydr Res 29:397–403.Google Scholar
  15. 15.
    Takahashi R, Kusakabe I, Maekawa A, Suzuki, Murakami K (1983)Japan J Trop Agr 27:140–148.Google Scholar
  16. 16.
    Kaufman BT (1974)Method in Enzymology 34:272–281. Academic Press, New York.Google Scholar
  17. 17.
    Hakomori S (1964)J Biochem 55:205–208.Google Scholar
  18. 18.
    Saito T, Itho T, Adachi S, Suzuki T, Usui T (1981)Biochim Biophys Acta 678:257–267.Google Scholar
  19. 19.
    Usui T, Mizuno T, Kato K, Tomoda M, Miyajima C (1979)Agric Biol Chem 43:863–865.Google Scholar
  20. 20.
    Ogawa K, Murata T, Usui T (1991)Carbohydr Res 212:289–294.Google Scholar
  21. 21.
    Palmer TN, Ryman BE, Whelan WJ (1976)Eur J. Biochem 69:105–115.Google Scholar
  22. 22.
    Kobayashi S, Kainuma K, Kawasaki T, Shoda S (1991)J Am Chem Soc 113:3079–3084.Google Scholar
  23. 23.
    Usui T, Hayashi Y, Nanjo F, Ishido Y (1988)Biochim Biophys Acta 952:179–184.Google Scholar
  24. 24.
    Usui T, Matsui H, Isobe K (1990)Carbohydr Res 203:65–77.Google Scholar

Copyright information

© Chapman & Hall 1994

Authors and Affiliations

  • Taichi Usui
    • 1
  • Masahiro Suzuki
    • 1
  • Toshinari Sato
    • 1
  • Hirokazu Kawagishi
    • 1
  • Kyoko Adachi
    • 2
  • Hiroshi Sano
    • 2
  1. 1.Faculty of Agriculture, Department of Applied Biological ChemistryShizuoka UniversityShizuokaJapan
  2. 2.Marine Biotechnology Institute Co., LtdShimizuJapan

Personalised recommendations