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Journal of Fluorescence

, Volume 5, Issue 2, pp 231–235 | Cite as

A long-wavelength biolabeling reagent based on the oxonol fluorophore

  • Philip L. Southwick
  • Klaus M. Hahn
  • Jean Chao
  • Patrick L. Perry
  • Allan S. Wagman
  • Marc Wagner
  • Alan S. Waggoner
Regular Articles

Abstract

A red fluorescent dye of the oxonol class, bis-[1-(carboxymethyl)barbituric acid-(5)]-pentamethinoxonol, has been synthesized and, in the form of the succinimidyl active ester, has been applied to antibody labeling for application to flow cytometry and to imaging of tissue sections. The new dye, named CMOX (for carboxymethyloxonol), shows maximum excitation at 583 nm and emission at 611 nm, with a quantum yield of 0.2 in aqueous buffer and methanol. Antibodies labeled with the new dye show favorable brightness, photostability, and low levels of nonspecific binding.

Key words

Oxonol succinimidyl ester fluorescent labeling flow cytometry immunofluorescence 

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Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • Philip L. Southwick
    • 1
  • Klaus M. Hahn
    • 2
  • Jean Chao
    • 1
  • Patrick L. Perry
    • 1
  • Allan S. Wagman
    • 1
  • Marc Wagner
    • 1
  • Alan S. Waggoner
    • 1
  1. 1.Center for Light Microscope Imaging and BiotechnologyCarnegie Mellon UniversityPittsburgh
  2. 2.Division of Virology, Department of NeuropharmacologyScripps Research InstituteLa Jolla

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