Journal of Fluorescence

, Volume 5, Issue 1, pp 39–50

Determination of DPH order parameters in unoriented vesicles

  • D. Toptygin
  • L. Brand
Special Topic Articles

DOI: 10.1007/BF00718781

Cite this article as:
Toptygin, D. & Brand, L. J Fluoresc (1995) 5: 39. doi:10.1007/BF00718781


This article reviews the determination of orientational order parameters in non-macroscopically oriented membranes from the data obtained with the fluorescent probe all-trans-1,6-diphenyl-1,3,5-hexatriene (DPH). Special attention is paid to the effect of microheterogeneity in the probe environment on the recovered values of the order parameters. An effort is made to accommodate new findings in the existing picture of orientational order in membranes.

Key words

Fluorescence order parameters lipid membranes refractive index microheterogeneity diphenylhexatriene 

Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • D. Toptygin
    • 1
  • L. Brand
    • 1
  1. 1.McCollum-Pratt Institute, Department of BiologyJohns Hopkins UniversityBaltimore

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