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Plant Cell Reports

, Volume 8, Issue 2, pp 124–127 | Cite as

Somatic embryogenesis and plant regeneration from cell suspension and tissue cultures of mature himalayan poplar (Populus ciliata)

  • Gurvinder S. Cheema
Article

Abstract

Somatic embryogenesis and plantlet formation were obtained from callus and cell suspension cultures of 40-year- old Himalayan Poplar (Populus ciliata Wall ex Royle). Callus and cell suspensions were obtained by transfer of inoculum of semiorganized leaf cultures, which were maintained on Murashige and Skoog (MS) medium supplemented with benzylaminopurine (BAP), to MS with 2,4-dichlorophenoxyacetic acid (2,4-D). Reduction of 2,4-D concentration during subsequent subculture of cell suspensions resulted in the formation of embryoids. These embryoids developed further only after being transferred to agar-based MS medium supplemented with BAP and naphthalene acetic acid. Loss of embryogenic potential was observed in cell suspensions after 6 subcultures. However, callus cultures retained the embryogenic potential even after repeated subcultures for more than a year. Plantlets could be successfully hardened and grown in natural outdoor conditions.

Key Words

Populus ciliata somatic embryogenesis plant regeneration 

Abbreviations

BAP

6-benzylaminopurine

2,4-D

2,4-dichlorophenoxyacetic acid

NAA

1-naphthalene acetic acid

MS

Murashige and Skoog (1962) medium

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Gurvinder S. Cheema
    • 1
  1. 1.Tata Energy Research InstituteNew DelhiIndia

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