Plant Cell Reports

, Volume 8, Issue 3, pp 133–136

Genotype specificity of the somatic embryogenesis response in cotton

  • Norma L. Trolinder
  • Chen Xhixian
Article

Summary

Thirty eight cultivars, strains, and races ofGossypium were screened for somatic embryogenesis with the protocols developed as a model forG. hirsutum L. cv. Coker 312. Four classes of response were identified; high, moderate, low, and non-embryogenic. Four cultivars were further screened with 13 growth regulator regimes to determine if culture environment could change the classification or induce a higher level of response. The classification or level of response did not change. Screening of individual seedlings within a cultivar indicated that genotypic variation for embryogenesis existed. Highly embryogenic individuals were selected from cvs. Coker 312 and Paymaster 303 for use as germplasm sources for transfer of the embryogenic trait to other cultivars and genetic stocks. Only genetically responsive genotypes are amenable to the model developed for Coker 312.

Key words

Cotton somatic embryogenesis cultivar 

Abbreviations

2,4-D

2,4-dichlorophenoxyacetic acid (Carolina Biological)

2iP

N6-(2-iso-pentenyl) adenine (Sigma)

NAA

A-naphthaleneacetic acid (Sigma)

K

kinetin (Sigma)

IE

Index of embryogenesis

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Norma L. Trolinder
    • 1
  • Chen Xhixian
    • 1
  1. 1.Plant Physiologist and Visiting ScientistUSDA-ARSLubbockUSA

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