Skip to main content
Log in

Selection of bacterial wilt-resistant tomato through tissue culture

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Bacterial wilt-resistant plants were obtained using a tomato tissue culture system. A virulent strain ofPseudomonas solanacearum secreted some toxic substances into the culture medium. Leaf explant-derived callus tissues which were resistant to these toxic substances in the culture filtrate were selectedin vitro and regenerated into plants. These plants expressed bacterial wilt resistance at the early infection stage to suppress or delay the growth of the inoculated bacteria. On the other hand, complete resistance was obtained in self-pollinated progeny of regenerants derived from non-selected callus tissues. These plants showed a high resistance when inoculated with this strain, and were also resistant when planted in a field infested with a different strain of the pathogen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Akiyama Y, Eda S, Nishikawaji S, Tanaka H, Fujimori T, Kato K, Ohnishi A (1986) Agric Biol Chem 50:747–751

    Google Scholar 

  • Brettell RIS, Ingram DM (1979) Biol Rev 54:329–345

    Google Scholar 

  • Carlson PS (1973) Science 180:1366–1368

    Google Scholar 

  • Evans DA, Sharp WR (1983) Science 221:949–951

    Google Scholar 

  • Husain A, Kelman A (1958) Phytopathology 48:155–165.

    Google Scholar 

  • Larkin PJ, Scowcroft WR (1981) Theor Appl Genet 60:197–214

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol Plant 15:473–497

    Google Scholar 

  • Shahin EA, Spivey R (1986) Theor Appl Genet 73:164–169

    Google Scholar 

  • Sunderland N (1977)In Plant Tissue and Cell Culture (Street HE,ed), Blackwell Scientific Publications, Oxford, pp 177–205

    Google Scholar 

  • Toyoda H, Tanaka N, Hirai T (1984) Ann Phytopath Soc Japan 50:53–62

    Google Scholar 

  • Toyoda H, Ogata H, Matsuda Y, Chatani K, Hirai T (1985) Plant Tissue Culture Letters 2:70–73

    Google Scholar 

  • Toyoda H, Hashinoto H, Utsumi R, Kobayashi H, Ouchi S (1988) Phytopathology 78:1307–1311.

    Google Scholar 

  • Widholm JM (1972) Stain Technol 47:189–194

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by J. M. Widholm

Rights and permissions

Reprints and permissions

About this article

Cite this article

Toyoda, H., Shimizu, K., Chatani, K. et al. Selection of bacterial wilt-resistant tomato through tissue culture. Plant Cell Reports 8, 317–320 (1989). https://doi.org/10.1007/BF00716663

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00716663

Keywords

Navigation