Abstract
Bacterial wilt-resistant plants were obtained using a tomato tissue culture system. A virulent strain ofPseudomonas solanacearum secreted some toxic substances into the culture medium. Leaf explant-derived callus tissues which were resistant to these toxic substances in the culture filtrate were selectedin vitro and regenerated into plants. These plants expressed bacterial wilt resistance at the early infection stage to suppress or delay the growth of the inoculated bacteria. On the other hand, complete resistance was obtained in self-pollinated progeny of regenerants derived from non-selected callus tissues. These plants showed a high resistance when inoculated with this strain, and were also resistant when planted in a field infested with a different strain of the pathogen.
Similar content being viewed by others
References
Akiyama Y, Eda S, Nishikawaji S, Tanaka H, Fujimori T, Kato K, Ohnishi A (1986) Agric Biol Chem 50:747–751
Brettell RIS, Ingram DM (1979) Biol Rev 54:329–345
Carlson PS (1973) Science 180:1366–1368
Evans DA, Sharp WR (1983) Science 221:949–951
Husain A, Kelman A (1958) Phytopathology 48:155–165.
Larkin PJ, Scowcroft WR (1981) Theor Appl Genet 60:197–214
Murashige T, Skoog F (1962) Physiol Plant 15:473–497
Shahin EA, Spivey R (1986) Theor Appl Genet 73:164–169
Sunderland N (1977)In Plant Tissue and Cell Culture (Street HE,ed), Blackwell Scientific Publications, Oxford, pp 177–205
Toyoda H, Tanaka N, Hirai T (1984) Ann Phytopath Soc Japan 50:53–62
Toyoda H, Ogata H, Matsuda Y, Chatani K, Hirai T (1985) Plant Tissue Culture Letters 2:70–73
Toyoda H, Hashinoto H, Utsumi R, Kobayashi H, Ouchi S (1988) Phytopathology 78:1307–1311.
Widholm JM (1972) Stain Technol 47:189–194
Author information
Authors and Affiliations
Additional information
Communicated by J. M. Widholm
Rights and permissions
About this article
Cite this article
Toyoda, H., Shimizu, K., Chatani, K. et al. Selection of bacterial wilt-resistant tomato through tissue culture. Plant Cell Reports 8, 317–320 (1989). https://doi.org/10.1007/BF00716663
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00716663