Sexual Plant Reproduction

, Volume 5, Issue 1, pp 57–63 | Cite as

Effect of electrical fields and external ionic currents on pollen-tube orientation

  • R. Malhó
  • J. A. Feijó
  • M. S. S. Pais
Original Articles

Summary

Agapanthus umbelatus pollen tubes (PTs) display a number of different growth patterns when germinated in an electric field of 750 mV· mm−1. When pollen is germinated near the cathode (82.44% of orientation to the cathode side) or near the anode (55.35% of orientation to the anode), growth is oriented parallel to the applied field but when germinated at an intermediate position, there is random growth. An increase and decrease in the orientation rates as well as reversion of the polarized growth were observed when the growth conditions were systematically altered. These findings reflect the influence of different ionic currents present in the germination medium. These ionic currents induce the formation of ionic gradients, which were monitored by ion-HPLC. The individual omission of Ca2+, K+, Mg2+ and Cl suppresses or alters the oriented growth pattern. The presence of ionic gradients is not by itself suficient to trigger the polarization of tube growth as the presence of an electric field which drives the ionic currents is essential for this to occur.

Key words

Agapanthus umbelatus Electric fields Ionic currents Polarized growth Pollen tube 

Abbreviations

PT

Pollen tube

DNS

3,5-dinitro salycilic acid;

TP

transient polarization

HPLC

high precision liquid chroma tography

DC

direct current

References

  1. Armstrong CM, Matteson DR (1986) The role of calcium ions in the closing of K channels. J Gen Physiol 87:817–832Google Scholar
  2. Bacic A, Delmer DP (1981) Stimulation of membrane-associated polyssacharide synthetases by a membrane potential in developing cotton fibers. Planta 152:346–351Google Scholar
  3. Bednarska E (1989) The effect of exogenous Ca2+ ions on pollen grain germination and pollen tube growth. Investigations with 45Ca2+ together with verapamil, La3+, and ruthenium Red. Sex Plant Reprod 2:53–58Google Scholar
  4. Brewbaker JL, Kwack BH (1963) The essential role of calcium ion in pollen germination and pollen tube growth. Am J Bot 50:859–865Google Scholar
  5. Burgess J, Linstead PJ (1982) Cell-wall differentiation during growth of electrically polarised protoplasts ofPhyscomitrella. Planta 156:241–248Google Scholar
  6. Bush DR, Sze H (1986) Calcium transport in tonoplast and endoplasmic reticulum vesicles isolated from cultured carrot cells. Plant Physiol 80:549–555Google Scholar
  7. Chanson A, McNaughton E, Taiz L (1984) Evidence for a KCl-stimulated, Mg2+ -ATPase on the golgi of corn coleoptiles. Plant Physiol 76:498–507Google Scholar
  8. Chen T-H, Jaffe LF (1979) Forced calcium entry and polarized growth ofFunaria spores. Planta 144:401–406Google Scholar
  9. Churchill KA, Sze H (1983) Anion-sensitive, H+-pumping ATPase in membrane vesicles from oat roots. Plant Physiol 71:610–617Google Scholar
  10. Davis RF (1981) Electrical properties of the plasmalemma and tonoplast inValonia ventricosa. Plant Physiol 67:825–831Google Scholar
  11. De Loof A (1986) The electrical dimension of the cells: the cell as a miniature electrophoresis chamber. Int Rev Cytol 104:251–352Google Scholar
  12. Hager A, Berthold W, Biber W, Edel H-G, Lanz C, Schiebel G (1986) Primary and secondary energized ion translocating systems on membranes of plant cells. Ber Dtsch Bot Ges 99:281–295Google Scholar
  13. Heslop-Harrison JS, Reger BJ (1986) Chloride and potassium ions and turgidity in the grass stigma. J Plant Physiol 124:55–60Google Scholar
  14. Jaffe LA, Weisenseel MH, Jaffe LF (1975) Calcium accumulations within the growing tips of pollen tubes. J Cell Biol 67:488–492Google Scholar
  15. Jiao Xin-zhi, Ni Jin-shan, Li Wei-lin, Wang Yan-zhi (1988) Comparative studies on vacuolar membrane, mitochondrial and plasma membrane ATPases or rice and oat roots. Acta Biol Exp Sin 21:409–416Google Scholar
  16. Mascarenhas JP (1975) The biochemistry of angiosperm pollen development. Bot Rev 41:259–314Google Scholar
  17. Picton JM, Steer MW (1983) Evidence for the role of Ca2+ ions in tip extension in pollen tubes. Protoplasma 115:11–17Google Scholar
  18. Rasi-Caldogno F, Pugliarello MC, De Michelis MI (1987) The Ca2+transport ATPase of plant plasma membrane catalyzes an H+/Ca2+ exchange. Plant Physiol 83:994–1000Google Scholar
  19. Rathore KS, Hodges TK, Robinson KR (1988) A refined technique to apply electrical currents to callus cultures. Plant Physiol 88:515–517Google Scholar
  20. Reinhold L, Kaplan A (1984) Membrane transport of sugars and amino acids. Annu Rev Plant Physiol 35:45–83Google Scholar
  21. Reiss H-D, Herth W (1985) Nifedipine-sensitive calcium channels are involved in polar growth of Lily pollen tubes. J Cell Sci 76:247–254Google Scholar
  22. Robinson KR (1985) The response of cells to electrical fields: a review. J Cell Biol 101:2023–2027Google Scholar
  23. Robinson KR, Jaffé LF (1975) Polarizing fucoid eggs drive a calcium current through themselves. Science 187:70–72Google Scholar
  24. Sanders D (1980) The mechanism of Cl transport at the plasma membrane ofChara corallina. I. Cotransport with H+. J Membr Biol 53:129–141Google Scholar
  25. Schnepf E (1986) Cellular polarity. Annu Rev Plant Physiol 37:23–47Google Scholar
  26. Sperber D (1984) Das Wachstum Pflanzlicher Zellen und Organe im Magnetischen und Elektrischen Feld. Konstanzer Dissertationen Nr. 51. Konstanz, Hartung, GorreGoogle Scholar
  27. Sperber D, Dransfeld K, Maret G, Weisenseel MH (1981) Oriented growth of pollen tubes in strong magnetic fields. Naturwissenschaften 68:40–41Google Scholar
  28. Steer MW, Steer JW (1989) Pollen tube tip growth. New Phytol 23:323–358Google Scholar
  29. Sumner JR, Somers GF (1944) Laboratory experiments in biological chemistry. Academic Press, New YorkGoogle Scholar
  30. Sze H (1985) H+-translocating ATPases, advances using membrane vesicles. Annu Rev Plant Physiol 36:175–208Google Scholar
  31. Thaler M, Steigner W, Forster B, Kohler K, Simonis W, Urbach W (1989) Calcium activation of potassium channels in the plasmalemma ofEremosphaera viridis. J Exp Bot 40:1195–1203Google Scholar
  32. Wagner GJ, Lin W (1982) An active proton pump of intact vacuoles isolated from Tulipa petals. Biochem Biophys Acta 689:261–266Google Scholar
  33. Walker RR, Leigh RA (1981) Characterization of a salt-stimulated ATPase activity associated with vacuoles isolated from storage roots of Red Beet (Beta vulgaris L.). Planta 153:140–149Google Scholar
  34. Weisenseel MH, Jaffé LF (1976) The major growth current through Lily pollen tubes enters as K+ and leaves as H+. Planta 133:1–7Google Scholar
  35. Weisenseel MH, Kicherer RM (1981) Ionic currents as control mechanisms in cytomorphogenesis. In: Kiermayer O (ed) Cytomorphogenesis in plants, vol 8. Springer, Berlin Heidelberg New York, pp 379–399Google Scholar
  36. Weisenseel MH, Nuccitelli R, Jaffe LF (1975) Large electrical currents traverse growing pollen tubes. J Cell Biol 66:556–567Google Scholar
  37. Williams LE, Hall JL (1989) ATPase and proton-translocating activities in a plasma membrane-enriched fraction from cotyledons ofRicinus communis. J Exp Bot 40:1205–1213Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • R. Malhó
    • 1
  • J. A. Feijó
    • 1
  • M. S. S. Pais
    • 1
  1. 1.Departamento de Biologia VegetalFaculdade de Ciências da Universidade de Lisboa, R. Ernesto de VasconcelosLisboaPortugal

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