Chromosome Research

, Volume 3, Issue 8, pp 466–472 | Cite as

Analysis of rye B-chromosome structure using fluorescencein situ hybridization (FISH)

  • Timothy M. Wilkes
  • Michael G. Francki
  • Peter Langridge
  • Angela Karp
  • R. Neil Jones
  • John W. ForsterEmail author


Fluorescencein situ hybridization (FISH) has been used to analyse the structure of the rye B chromosome. Genomicin situ hybridization (GISH) demonstrates the high level of overall similarity between A and B chromosomes of rye, as well as the presence of a number of specific sequences. The B-specific repeat families D1100 and E3900 have been analysed in terms of their physical location and possible contiguity. Rye Bs contain members of the rye-specific dispersed repetitive family R173, as well as centromeric regions similar to those of the As. The B chromosomes analysed in our study lack detectable rDNA sequences. Anomalous results have been obtained with a number of subtelomeric repetitive probes from rye. Bs usually lack these sequences, but evidence is presented that in some cases A–B translocation events may relocate such sequences from the As to the Bs. These data are discussed in the context of current models for the origin of the B chromosome.

Key words

B chromosome DNA sequence composition fluorescencein situ hybridization repetitive sequence Secale cereale translocations 


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Copyright information

© Rapid Science Publishers 1995

Authors and Affiliations

  • Timothy M. Wilkes
    • 1
  • Michael G. Francki
    • 2
  • Peter Langridge
    • 2
  • Angela Karp
    • 3
  • R. Neil Jones
    • 1
  • John W. Forster
    • 1
    Email author
  1. 1.the Genetics Group, Institute of Biological SciencesUniversity of WalesAberystwythUK
  2. 2.the Department of Plant Sciences, Waite Agricultural Research InstituteUniversity of AdelaideAustralia
  3. 3.the IACR — Long Ashton Research Station, Department of Agricultural SciencesUniversity of BristolLong Ashton, BristolUK

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