Advertisement

Chromosome Research

, Volume 3, Issue 8, pp 466–472 | Cite as

Analysis of rye B-chromosome structure using fluorescencein situ hybridization (FISH)

  • Timothy M. Wilkes
  • Michael G. Francki
  • Peter Langridge
  • Angela Karp
  • R. Neil Jones
  • John W. Forster
Article

Abstract

Fluorescencein situ hybridization (FISH) has been used to analyse the structure of the rye B chromosome. Genomicin situ hybridization (GISH) demonstrates the high level of overall similarity between A and B chromosomes of rye, as well as the presence of a number of specific sequences. The B-specific repeat families D1100 and E3900 have been analysed in terms of their physical location and possible contiguity. Rye Bs contain members of the rye-specific dispersed repetitive family R173, as well as centromeric regions similar to those of the As. The B chromosomes analysed in our study lack detectable rDNA sequences. Anomalous results have been obtained with a number of subtelomeric repetitive probes from rye. Bs usually lack these sequences, but evidence is presented that in some cases A–B translocation events may relocate such sequences from the As to the Bs. These data are discussed in the context of current models for the origin of the B chromosome.

Key words

B chromosome DNA sequence composition fluorescencein situ hybridization repetitive sequence Secale cereale translocations 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Appels R, Driscoll C, Peacock WJ (1978) Heterochromatin and highly repeated DNA sequences in rye (Secale cereale).Chromosoma 70: 67–89.Google Scholar
  2. Appels R, Dennis ES, Smyth DR, Peacock WJ (1981) Two repeated DNA sequences from the heterochromatic regions of rye (Secale cereale).Chromosoma 84: 265–277.Google Scholar
  3. Bedbrook JR, Gerlach W, Thompson R, Jones J, Flavell RB (1980) A molecular description of telomeric heterochromatin inSecale species.Cell 19: 545–560.Google Scholar
  4. Blunden R (1994) Molecular analysis of the rye B-chromosome. PhD thesis, University of Wales.Google Scholar
  5. Blunden R, Wilkes TM, Forster JWet al. (1993) Identification of the E3900 family, a second family of rye B-chromosome specific repeated sequences.Genome 36: 706–711.Google Scholar
  6. Close TJ, Rodriguez RL (1982) Construction and characterization of the chloramphenicol resistant cartridge: a new approach to the transcriptional mapping of extrachromosomal elements.Gene 20: 305–316.Google Scholar
  7. Cuadrado A, Jouve N (1994) Highly repetitive sequences in B-chromosomes ofSecale cereale revealed by fluorescencein situ hybridization.Genome 37: 709–712.Google Scholar
  8. Flavell RB, Rimpau J (1975) Ribosomal RNA genes and supernumerary B-chromosomes of rye.Heredity 35: 127–131.Google Scholar
  9. Francki MG, Langridge P (1994) The molecular identification of the midget chromosome from the rye genome.Genome 37: 1056–1061.Google Scholar
  10. Gerlach WL, Bedbrook JR (1979) Cloning and characterisation of ribosomal RNA genes from wheat and barley.Nucleic Acids Res 7: 1869–1885.Google Scholar
  11. Guidet F, Rogowsky PM, Taylor C, Song W, Langridge P (1991) Cloning and characterisation of a new rye-specific repeated sequence.Genome 34: 81–87.Google Scholar
  12. Jones JDG, Flavell RB (1982) The structure, amount and chromosomal localization of defined repeated sequences in species of the genusSecale.Chromosoma 86: 613–641.Google Scholar
  13. Jones RN, Puertas MJ (1993)The B-chromosomes of Rye, SC Verma commemorative volume. Chandrigarh, India: Punjab University.Google Scholar
  14. Jones RN, Rees H (1982)B-chromosomes. London: Academic Press.Google Scholar
  15. Kishikawa H (1965) Cytogenetic studies of B-chromosomes in rye,Secale cereale L., in Japan.Agric Bull Saga Univ 21: 1–81.Google Scholar
  16. Kota RS, Gill BS, Hulbert SH (1994) Presence of rye-specific repeated DNA sequences on the midget chromosome of rye.Genome 37: 619–624.Google Scholar
  17. McIntyre CL, Pereira S, Moran LB, Appels R (1990) NewSecale cereale (rye) DNA derivatives for the detection of rye chromosome segments in wheat.Genome 33: 635–640.Google Scholar
  18. Maluszynska J, Schweitzer D (1989) Ribosomal RNA genes in B-chromosomes ofCrepis capillaris detected by non-radioactivein situ hybridization.Heredity 62: 59–65.Google Scholar
  19. Murata M, Nakata N, Yasamuro Y (1992) Origin and molecular structure of a midget chromosome in a common wheat carrying rye cytoplasm.Chromosoma 102: 27–31.Google Scholar
  20. Nakata N, Asami H, Yasamuro Y, Sasaki M (1988) Role and transmission of midget chromosome found in rye cytoplasmic Chinese Spring line. Proceedings of the 7th International Wheat Genetics Symposium, pp 391–396.Google Scholar
  21. Pan WH, Houben A, Schlegel R (1993) Highly effective cell synchronization in plant roots by hydroxyurea and amiphos-methyl or colchicine.Genome 36: 387–390.Google Scholar
  22. Peeters JP, Griffiths AJF, Wilkes G (1985)In vivo karyotypic modifications following spontaneous cell fusion in maize (Zea mays L.).Can J Genet Cytol 27: 580–585.Google Scholar
  23. Pohler W, Schlegel R (1990) A rye plant with frequent A-B chromosome pairing.Hereditas 112: 217–220.Google Scholar
  24. Raeder U, Broda R (1985) Rapid preparation of DNA from filamentous fungi.Lett Appl Microbiol 1: 17–20.Google Scholar
  25. Rimpau J, Flavell RB (1975) Characterization of rye B-chromosome DNA by DNA-DNA hybridization.Chromosoma 52: 207–217.Google Scholar
  26. Pogowsky P, Manning S, Liu J-Y, Langridge P (1991) The R173 family of rye-specific repetitive DNA sequences: a structural analysis.Genome 34: 88–95.Google Scholar
  27. Sandery MJ, Forster JW, Blunden R, Jones RN (1990) Identification of a family of repeated sequences on the rye B-chromosome.Genome 33: 908–913.Google Scholar
  28. Sapre AB, Deshpande DS (1987) Origin of B-chromosomes inCoix L. through interspecific hybridization.J Hered 78: 191–196.Google Scholar
  29. Schwarzacher T, Heslop-Harrison JS, Leitch AR (1993) DNA:DNAin situ hybridization: methods for light microscopy. In: Harris N, ed.Plant Cell Biology: A Practical Approach. New York: Oxford University Press.Google Scholar
  30. Timmis JN, Ingle J, Sinclair J, Jones RN (1975) The genomic quality of rye B-chromosomes.J Exp Bot 26: 367–378.Google Scholar
  31. Tsujimoto H, Niwa K (1992) DNA structure of the B-chromosome of rye revealed byin situ hybridization using repetitive sequences.Jpn J Genet 67: 233–241.Google Scholar

Copyright information

© Rapid Science Publishers 1995

Authors and Affiliations

  • Timothy M. Wilkes
    • 1
  • Michael G. Francki
    • 2
  • Peter Langridge
    • 2
  • Angela Karp
    • 3
  • R. Neil Jones
    • 1
  • John W. Forster
    • 1
  1. 1.the Genetics Group, Institute of Biological SciencesUniversity of WalesAberystwythUK
  2. 2.the Department of Plant Sciences, Waite Agricultural Research InstituteUniversity of AdelaideAustralia
  3. 3.the IACR — Long Ashton Research Station, Department of Agricultural SciencesUniversity of BristolLong Ashton, BristolUK

Personalised recommendations