Antonie van Leeuwenhoek

, Volume 61, Issue 4, pp 323–332 | Cite as

Detection ofAspergillus andPenicillium extracellular polysaccharides (EPS) by ELISA: using antibodies raised against acid hydrolysed EPS

  • Henri J. Kamphuis
  • Gerhard A. De Ruiter
  • Gerrit H. Veeneman
  • Jacques H. van Boom
  • Frank M. Rombouts
  • Servé H. W. Notermans
Research Article
Received:
Accepted:

Abstract

Species of the fungal generaAspergillus andPenicillium produce immunologically active extracellular polysaccharides (EPS) in which galactofuranose residues are immunodominant. The antigenic determinant of the EPSA. fumigatus, A. niger andP. digitatum could be removed by acid hydrolysis. Due to the hydrolysis of the EPS the immunological reaction between IgG anti-native EPS and hydrolysed EPS disappeared. Antibodies raised in rabbits against the acid hydrolysed EPS revealed new antigenic determinants that were exposed as a result of the acid hydrolysis. Immunological inhibitory experiments showed that the antibodies were no longer directed to galactofuranose residues.

Enzyme Linked Immunosorbent Assay, carried out with antibodies raised against the acid hydrolysed EPS showed that the antibodies against the acid hydrolysed EPS were more species specific in comparison with the antibodies against the native EPS.

Key words

extracellular polysaccharides antibodies immuno-assay Aspergillus Penicillium 
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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Henri J. Kamphuis
    • 1
  • Gerhard A. De Ruiter
    • 1
  • Gerrit H. Veeneman
    • 3
  • Jacques H. van Boom
    • 3
  • Frank M. Rombouts
    • 1
  • Servé H. W. Notermans
    • 2
  1. 1.Department of Food ScienceWageningen Agricultural UniversityWageningenThe Netherlands
  2. 2.National Institute of Public Health and Environmental ProtectionBilthovenThe Netherlands
  3. 3.Gorlaeus LaboratoryLeidenThe Netherlands

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