Antonie van Leeuwenhoek

, Volume 61, Issue 4, pp 289–316

Contributions to a revision of the genusTrichosporon

  • E. Guého
  • M. Th. Smith
  • G. S. de Hoog
  • G. Billon-Grand
  • R. Christen
  • W. H. Batenburg van der Vegte
Research Article


The genusTrichosporon was revised using characters of morphology, ultrastructure, physiology, ubiquinone systems, mol% G+C of DNA, DNA/DNA reassociations and 26S ribosomal RNA partial sequences. A total of 101 strains was used, including all available type and authentic cultures of previously described taxa. Nineteen taxa could be distinguished, 15 of which having Q-9 coenzyme systems and 4 having Q-10. Sixteen previously described names were reduced to synonymy. One new species was described.

The genus is characterized by the presence of arthroconidia. Few species possess further diagnostic morphological characters, such as the presence of appressoria, macroconidia or meristematic conidiation. The septa of two species were found to be non-perforate, while those of the remaining species contained dolipores at variable degrees of differentiation, with or without vesicular or tubular parenthesomes. All species were able to assimilate a large number of carbon compounds; visible CO2 production was absent. The genus was found to be fairly homogeneous on the basis of a phylogenetic analysis of partial 26S rRNA sequences, with the exception ofT. pullulans which proved to be unrelated.

Most taxa were found to occupy well-defined ecological niches. Within the group of taxa isolated from humans, a distinction found be made between those involved in systemic mycoses and those which mainly caused public or non-public white piedras, respectively. One species was consistently associated with animals, while others came mainly from soil or water. One species was mesophilic and another psychrophilic.

Key words

Trichosporon white piedra systemic mycosis taxonomy nDNA rRNA ubiquinone systems basidiomycetous yeasts 


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  1. Aciole de Queiroz L (1973)Endomycopis montevidensis n. sp. Mycopath. Mycol. appl. 51: 307–314Google Scholar
  2. Akagi S (1929) Über eine neue mykotische Hautkrankheit ‘Trichosporia cutis psoriasiformis progressiva Asahi et Akagi’. Jap. J. Dermatol. Urol. 29: 53–55, 733–774Google Scholar
  3. Anaissie E, Bodey GP, Kantarsian H, Ro J, Vartivarian SE, Hopfer R, Hoy J & Rolston K (1989) New spectrum of fungal infections in patients with cancer. Rev. infect. Dis. 11: 369–378Google Scholar
  4. Arnold P & Ahearn DG (1972) The systematics of the genusPrototheca with a description of a new speciesP. filamenta. Mycologia 64: 265–275Google Scholar
  5. Avram A, Buyot G, Binet O, Gracia AM & Cesarini JP (1987) Étude clinique et mycologique concernant 11 cas de trichosporie noueuse (piedra blanche) génito-pubienne. Ann. Dermatol. Vénéréol. 114: 819–827Google Scholar
  6. Batista AC, Campos STC & Oliveira S (1963) Epidermomicose devida aTrichosporon loboi n. sp. Publ. Inst. Micol. Recife 207: 3–9Google Scholar
  7. Batista AC & Silveira JS (1959) Una variedade nova deEndomycopsis mali (Lewis) Dekker assinalada sôbre fezes humanas. Revta Assoc. Med. Bras. 5: 353–354Google Scholar
  8. Batista AC & Silveira JS (1960)Trichosporon figueirae n. sp. assinalado como fungo intestinal. Mycopath. Mycol. appl. 12: 196–198Google Scholar
  9. Behrend G (1890) Über Trichomycosis nodosa (Juhel-Rénoy): Piedra (Osorio). Berlin. klin. Wschr. 27: 464–467Google Scholar
  10. Berkhout CM (1923) De schimmelgeslachtenMonilia, Oidium, Oospora enTorula. Thesis, Utrecht.Google Scholar
  11. Billon-Grand G (1985) Co-enzyme Q de quelques espèces du genrePichia: détermination qualitative et quantitative. Mycopathologia 90: 101–106Google Scholar
  12. Boekhout T, Yamada Y, Weijman ACM, Roeymans HJ & Batenburg-van der Vegte WH (1992) The significance of coenzyme Q, carbohydrate composition and septal ultrastructure for the taxonomy of ballistospore-forming yeasts and fungi. Syst. Appl. Microbiol. 15: 1–11Google Scholar
  13. Ciferri R & Redaelli P (1935) Contribuzioni alla sistematica delle Torulopsidaceae. XV–XXXIII. Arch. Mikrobiol. 6: 9–72Google Scholar
  14. Cochet G (1940) Recherches sur la flore mycologique de l'intestin des rongeurs domestiques. Poulet-Malasis, AlençonGoogle Scholar
  15. DeArêa Leâo AE (1940) Consideraçôes sobre os Thallosporados. O gêneroTrichosporon. “Trichosporon minor” n. sp. produtor da piedra axilar. Mem. Inst. Oswaldo Cruz 35: 729–745Google Scholar
  16. DeBeurmann L & Gougerot H (1909) Les exascoses, saccharomycoses (mycose de Busse-Buschke) et parasaccharomycoses. Zymonématoses (mycose de Gilchrist). Parendomycoses et endomycoses (muguet). Bull. Mém. Soc. méd. Hôp. Paris 28: 250–263Google Scholar
  17. DeHoog GS (1979) The taxonomic position ofMoniliella, Trichosporonoides andHyalodendron-An essay. Stud. Mycol. 19: 81–90Google Scholar
  18. Diddens HA & Lodder J (1942) Die anaskosporogenen Hefen, zweite Hälfte. North-Holland, AmsterdamGoogle Scholar
  19. DoCarmo Sousa L & vanUden N (1967) Reisolation ofSarcinomyces inkin and its transfer to the genusTrichosporon. Mycologia 59: 653–657Google Scholar
  20. Fang SF, Yen TC & Yue JC (1966) Identification of some species ofGeotrichum. Acta Microbiol. Sin. 12: 64–73Google Scholar
  21. Fischman O, Pires de Camargo Z & Meireles MCA (1989) Genital white piedra: an emerging new fungal disease? 5th. Int. Conf. Mycoses, PAHO Sci. Publ. 396: 70–76Google Scholar
  22. Frágner P (1969)Trichosporon jirovecii sp. nov. Ceská Mykol. 23: 160–162Google Scholar
  23. Gams W & Domsch KH (1969) Bemerkungen zu einigen schwer bestimmbaren Bodenpilzen. Nova Hedwigia 18: 1–29Google Scholar
  24. Goto S, Sugiyama J & Iizuka H (1969) A taxonomic study of Antarctic yeasts. Mycologia 61: 748–774Google Scholar
  25. Guadet J, Julien J, Lafay JF, Brygoo Y (1989) Phylogeny of someFusarium species, as determined by large subunit rRNA sequence comparison. Mol. Biol. Evol. 6: 227–242Google Scholar
  26. Guého E, Kurtzman CP & Peterson SW (1989) Evolutionary affinities of heterobasidiomycetous yeasts estimated from 18S and 25S ribosomal RNA sequence divergence. System. Appl. Microbiol. 12: 230–236Google Scholar
  27. Guého E, de Hoog GS & Smith MTh (1992) Typification of the genusTrichosporon. Antonie van Leeuwenhoek 61: 000-000 (this issue)Google Scholar
  28. Guého E, Tredick J & Phaff HJ (1984) DNA base composition and DNA relatedness among species ofTrichosporon Behrend. Antonie van Leeuwenhoek 50: 17–32Google Scholar
  29. Hara N, Tubota Y, Saito K & Suto T (1989) Biochemical characteristics and ubiquinone of 44 strains ofTrichosporon beigelii and related organisms. J. gen. appl. Microbiol. 35: 1–10Google Scholar
  30. Hedrick LR & Dupont PD (1968) Two new yeasts:Trichosporon aquatile andTrichosporon eriense spp. n. Antonie van Leeuwenhoek 34: 474–482Google Scholar
  31. Kaplan W (1959) Piedra in lower animals. A case report of white piedra in a monkey and a review of the literature. J. Amer. Vet. Med. Ass. 134: 113–117Google Scholar
  32. Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16: 111–120Google Scholar
  33. King DS & Jong SC (1975)Sarcinosporon: A new genus to accommodateTrichosporon inkin andPrototheca filamenta. Mycotaxon 3: 89–94Google Scholar
  34. Kreger-van Rij NJW (Ed) (1984) The Yeasts, a Taxonomic Study. Elsevier, AmsterdamGoogle Scholar
  35. Kreger-van Rij NJW & Veenhuis M (1971) Septal pores inTrichosporon cutaneum. Sabouraudia 9: 36–38.Google Scholar
  36. Kurtzman CP, Smiley MJ, Johnson CJ, Wickerham LJ & Fuson GB (1980) Two new and closely related heterothallic species,Pichia amylophila andPichia mississippiensis: characterization by hybridization and deoxyribonucleic acid reassociation. Int. J. syst. Bact. 30: 208–216Google Scholar
  37. Kuttin ES & Müller J (1981)Trichosporon beemeri sp. nov. Antonie van Leeuwenhoek 47: 255–265Google Scholar
  38. Kwon-Chung KJ & Popkin TJ (1976) Ultrastructure of septal complex inFilobasidiella neoformans (Cryptococcus neoformans). J. Bacteriol. 126: 524–528Google Scholar
  39. Langeron M & Talice RV (1932) Nouvelles méthodes d'étude et essai de classification des champignons levuriformes. Annls Parasit. hum. comp. 10: 1–80Google Scholar
  40. Lasagni A & Ermacora E (1977) Su un caso di piedra blanca in Lombardia. Minerva Dermatol. 112: 241–243Google Scholar
  41. Leblond V, Saint Jean O, Datry A, Lecso G, Frances C, Bellefich S, Gentilini M & Binet JL (1986) Systemic infections withTrichosporon beigelii (cutaneum). Cancer 58: 2399–2405Google Scholar
  42. Lindner P (1895) Mikroskopische Betriebskontrolle in den Gärungsgewerben mit einer Einführung in die Hefenreinkultur, Infektionslehre and Hefenkunde. Parey, BerlinGoogle Scholar
  43. Londeron AT, Ramos CD & Fishman O (1966) White piedra of unusual localization. Sabouraudia 5: 132–133Google Scholar
  44. Maccecchini HL, Rudin Y, Blobel G, Schatz G (1979) Import of proteins into mitochondria: precursor forms of the extramitochondrially made Fl-ATPase subunits in yeast. Proc. Natl. Acad. Sci. U.S.A. 76: 343–347Google Scholar
  45. Manabe T, Moriya T, Shirabe T & Takemoto Y (1987) DisseminatedTrichosporon beigelii infection in a patient with malignant histiocytosis. Acta Pathol. Jpn 36: 1241–1250Google Scholar
  46. Marmur J & Doty P (1962) Determination of the base composition of deoxyribonucleic acid from thermal denaturation temperature. J. molec. Biol. 5: 109–118Google Scholar
  47. Martínez AT (1979) Ultrastructure ofMoniliella, Trichosporonoides andHyalodendron. Stud. Mycol. 19: 50–57Google Scholar
  48. McManus EJ & Jones JM (1985) Detection of aTrichosporon beigelii capsular polysaccharide in serum from a patient with disseminatedTrichosporon infection. J. clin. Microbiol. 21: 681–685Google Scholar
  49. Michot B & Bachellerie JP (1987) Comparisons of large subunit rRNAs reveal some eukaryote-specific elements of secondary structure. Biochimie 69: 11–253Google Scholar
  50. Michot B, Hassouna N & Bachellerie JP (1984) Secondary structure of mouse 28S rRNA and general model for the folding of the large rRNA in eukaryotes. Nucleic Acids Res. 12: 4259–4279Google Scholar
  51. Moore M (1935) Head infection caused by a newHemispora: H. coremiformis. Annls Missouri bot. Gdn 22: 317–334Google Scholar
  52. Moore RT (1978) Septal ultrastructure inSirobasidium magnum and its taxonomic implications. Antonie van Leeuwenhoek 45: 113–118Google Scholar
  53. Moore RT (1987) Micromorphology of yeasts and yeast-like fungi and its taxonomic implications. In: DeHoog GS et al. (Eds): The Expanding Realm of Yeast-like Fungi (pp. 203–226). Elsevier Sci. Publ., AmsterdamGoogle Scholar
  54. Moore RT & Kreger-van Rij (1972) Ultrastructure ofFilobasidium Olive. Can. J. Microbiol. 18: 1949–1951Google Scholar
  55. Morenz J (1963)Geotrichum candidum Link. Taxonomie, Diagnose und medizinische Bedeutung. Mykol. SchrReihe 1: 1–79Google Scholar
  56. Moses A & Vianna G (1913) Sobra nova micose humana, causada por cogumelo ainda nâo descrito:Proteomyces infestans. Mem. Inst. Oswaldo Cruz 5: 192–210Google Scholar
  57. Nakase T (1971) New species of yeasts found in Japan. J. gen. appl. Microbiol 17: 409–419Google Scholar
  58. Oho O (1919) Ueber das sogenannte “Inkin”. Kyoto Igaku Zasshi 16: 7–21Google Scholar
  59. Ota M (1926a) Sur quelques champignons pathogènes du typeTrichosporon beigeli Vuillemin. Annls Parasit. hum. comp. 4: 1–13Google Scholar
  60. Ota M (1926b) Champignons parasites de l'homme. Japan. J. Dermatol. Urol. 26: 111–142Google Scholar
  61. Pereiro Miguens M (1952) Piedra blanca europea (primer caso descrito en España). Actas Derm. Sif. 43: 537–545Google Scholar
  62. Pereiro Miguens M & Pereiro Ferreiros M (1987) Un caso de piedra blanca en un perro. Rev. Ibér. Micol. 4: 69–76Google Scholar
  63. Phaff HJ, Mrak EM & Williams OB (1952) Yeasts isolated from shrimp. Mycologia 44: 431–451Google Scholar
  64. Pore RS, d'Amato RF & Ajello L (1977)Fissuricella gen. nov.: a new taxon forPrototheca filamenta. Sabouraudia 15: 69–70Google Scholar
  65. Rabenhorst L (1867) Zwei Parasiten an den todten Haaren der Chignons. Hedwigia 6: 49Google Scholar
  66. Ramírez C (1957) Contribucion al estudio de la ecologia de las levaduras I. Estudio de levaduras aisladas de hongos carnosos. Microbiol. Esp. 10: 215–247Google Scholar
  67. Rischin M (1921) Ueber einen Fall von bisher noch nicht beschriebenerParendomyces-Erkrankung, die unter dem Bilde der tiefen (Sycosis parasitaria) und oberflächlichen Trichophytie verlief. Arch. Dermat. Syphil. 134: 232–242Google Scholar
  68. Saëz H (1964)Geotrichum vanriji nouvelle espèce de champignon arthrosporé isolé chez une sarcelle d'été (Anas querquedula L.). Bull. mens. Soc. Linn. Lyon 33: 265–270Google Scholar
  69. Saëz H (1977)Trichosporon lutetiae, nouvelle espèce de levure isolée chez un cerf d'eld de Thaïlande. Bull. mens. Soc. Linn. Lyon 46: 365–367Google Scholar
  70. Saitou N & Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molec. Biol. Evol. 4: 406–425Google Scholar
  71. Sarfati C, Basset D, Abdelmoula J, Sanson-Lepors MJ, Gluckman E, Devergie A, Guilmot F & Drouhet E (1983) Septicémie mortelle àTrichosporon cutaneum chez un sujet immunodéprimé. Bull. Soc. Fr. Mycol. méd. 12: 287–289Google Scholar
  72. Seidler RJ & Mandel M (1971) Quantitative aspects of ribonucleic acid renaturation: Base composition, state of chromosome replication, and polynucleotide homologies. J. Bact. 106: 608–614Google Scholar
  73. Smit J (1934) Uber die Ursachen des Aufblähens von Belebtschlamm. Arch. Microbiol. 5: 550–560Google Scholar
  74. Smith MTh & Batenburg-van der Vegte WH (1985) Ultrastructure of septa inBlastobotrys andSporothrix. Antonie van Leeuwenhoek 51: 121–128Google Scholar
  75. Stautz W (1931) Beiträge zur Schleimflussfrage. Phytopath. Z. 3: 163–229Google Scholar
  76. Stenderup A, Schoenheyder H, Ebbesen P & Melbye M (1986) White piedra andTrichosporon beigelii carriage in homosexual men. J. med. vet. Mycol. 24: 401–406Google Scholar
  77. Studier J.A. & Keppler K.J. (1988). A note on the neighborjoining algorithm of Saitou and Nei. Molec. Biol. Evol. 5: 729–731Google Scholar
  78. Surmont I, Vergauwen B, Marcelis L, Verbist L, Verhoef G & Boogaerts M (1990) First report of chronic meningitis caused byTrichosporon beigelli. Eur. J. clin. Microbiol. infect. Dis. 9: 226–229Google Scholar
  79. Swofford DL & Olsen GJ (1990) Phylogeny reconstruction. In: Hillis DM & Moritz C (Eds): Molecular Systematics (pp. 411–501). Sinauer Associates Inc.Google Scholar
  80. Van derWalt JP & Yarrow D (1984) Methods for the isolation, maintenance, classification and identification of yeasts. In: Kreger-Van Rij NJW (Ed). The Yeasts a Taxonomic Study. 3rd ed. (pp. 45–104). North-Holland, AmsterdamGoogle Scholar
  81. vanOorschot CAN (1980) A revision ofChrysosporium and allied genera. Stud. Mycol. 20: 1–89Google Scholar
  82. VanOorschot CAN & deHoog GS (1981) Dimorphic behaviour and taxonomy ofTrichosporiella sporotrichoides. Antonie van Leeuwenhoek 47: 353–366Google Scholar
  83. Vuillemin P (1902)Trichosporum et trichospories. Arch. Parasit. 5: 38–66Google Scholar
  84. Weigmann H & Wolff A (1909) Über einige zum ‘Rübengeschmack’ der Butter beitragende Mycelpilze. Centbl. Bakt. ParasitKde, Abt. 2, 22: 657–671Google Scholar
  85. Weijman ACM (1979) Carbohydrate composition and taxonomy ofGeotrichum, Trichosporon and allied genera. Antonie van Leeuwenhoek 45: 119–127Google Scholar
  86. Windisch S (1952) Zur Biologie und Systematik des Milchschimmels und einiger ähnlicher Formen II. Milchschimmelähnliche Pilze. Beitr. Biol. Pfl. 29: 143–170Google Scholar
  87. Windisch S (1965) Über zwei neue Pilzarten,Protendomycopsis domschii n. g., n. sp. undEndomycopsis laibachii n. sp. Beitr. Biol. Pfl. 41: 337–358Google Scholar
  88. Wolff M, Buré A, Legrand P, Poirot JL, Marche C, Laporte D & Drouhet E (1984) Septicémies mortelles àTrichosporon spp. chez trois patients immunodéprimés. Méd. Malad. infect. 14: 608Google Scholar
  89. Yamada Y, Nakazawa E & Kondo K (1982) The coenzyme Q system in strains ofTrichosporon species and related organisms. J. gen. appl. Microbiol. 28: 355–358Google Scholar
  90. Zaror L, Fischman O, Petri V, Carvalho MTF, Pope S & Porfirio N (1980) Piedra blanca genital. Reporte de tres casos. Boln Micol. 4: 125–127Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • E. Guého
    • 1
  • M. Th. Smith
    • 2
  • G. S. de Hoog
    • 3
    • 4
  • G. Billon-Grand
    • 5
  • R. Christen
    • 6
  • W. H. Batenburg van der Vegte
    • 7
  1. 1.Untté de MycologieInstitut PasteurParis 15France
  2. 2.Yeast Division, Centraalbureau voor SchimmelculturesDelft University of TechnologyDelftThe Netherlands
  3. 3.Centraalbureau voor SchimmelculturesBaarnThe Netherlands
  4. 4.Department of Molecular Cell BiologyUniversity of AmsterdamAmsterdamThe Netherlands
  5. 5.Institut de Chimie et Biologie Moléculaire et CellulaireUniversité Claude BernardVilleurbanne CedexFrance
  6. 6.Unité de Biologie Cellulaire MarineURA 671-CNRS-Paris 6Villefranche sur MerFrance
  7. 7.Department of Microbiology and EnzymologyDelft University of TechnologyDelftThe Netherlands

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