Antonie van Leeuwenhoek

, Volume 61, Issue 4, pp 269–276

Alterations in the cell wall ofSaccharomyces cerevisiae induced by the alpha sex factor or a mutation in the cell cycle

  • Silvia Díaz
  • Samuel Zínker
  • José Ruiz-Herrera
Research Article

Abstract

We performed experiments in parallel to study the rate of synthesis of cell wall polysaccharides and the activity of glycosyl transferases inSaccharomyces cerevisiae after arrest of acdc 28 mutant in G1 phase by either addition of alpha-factor or transfer to the non-permissive temperature. Both effectors brought about similar time-dependent increases in the rate of synthesis and deposition of the cell wall polysaccharides chitin, glucan and mannan. These changes in cell wall composition were accompanied by an increase in the specific activities of glucan and chitin synthetases. This increase was inhibited by cycloheximide suggesting that it representedde novo enzyme biosynthesis and not enzyme activation. Our data are consistent with the notion that both alpha-factor and thecdc 28 mutation affect the same stage-specific function that controls the temporal expression of glycosyl transferases.

Key words

Saccharomyces cerevisiae cell wall alpha-factor cdc mutant chitin glucan 

Abbreviations

GlcNAc

N-acetyl glucosamine

UDPGIcNAc

uridine-diphosphate-N-acetyl glucosamine

UDPGlc

uridine-diphosphate glucose

TCA

trichloroacetic acid

EDTA

ethylene diamino tetraacetate

TAME

tosyl-L-arginyl methyl ester

GTP

guanosine triphosphate

WGA

wheat germ agglutinin

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Silvia Díaz
    • 1
  • Samuel Zínker
    • 1
  • José Ruiz-Herrera
    • 1
    • 2
  1. 1.Departamento de Genética y Biología Molecular, Unidad Irapuato, Centro de Investigación y de Estudios AvanzadosInstituto Politécnico NacionalMéxico
  2. 2.Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios AvanzadosInstituto Politécnico NacionalMéxico
  3. 3.Departament de Microbiología, Facultat de FarmaciaUniversitat de ValenciaValencia (l'Horta)Spain

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