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Cellular and Molecular Neurobiology

, Volume 8, Issue 3, pp 259–268 | Cite as

Control of exocytosis from adrenal chromaffin cells

  • Ronald W. Holz
Review

Summary

  1. 1.

    Calcium-dependent exocytosis of catecholamines from intact and digitonin-permeabilized bovine adrenal chromaffin cells was investigated.

     
  2. 2.

    45Ca2+ uptake and secretion induced by nicotinic stimulation or depolarization in intact cells were closely correlated. The results provide strong support for Ca2+ entry being the trigger for exocytosis.

     
  3. 3.

    Experiments in which the H+ electrochemical gradient across the intracellular secretory granule (chromaffin granule) membrane was altered indicated that the gradient does not play an important role in exocytosis.

     
  4. 4.

    Ca2+ entry into the cells is associated with activation of phospholiphase C and a rapid translocation of protein kinase C to membranes.

     
  5. 5.

    The plasma membrane of chromaffin cells was rendered permeable to Ca2+, ATP, and proteins by the detergent digitonin without disruption of the intracellular secretory granules. In this system in which the intracellular milieu can be controlled, micromolar Ca2+ directly stimulated catecholamine secretion.

     
  6. 6.

    Treatment of the cells with phorbol esters and diglyceride, which activate protein kinase C, enhanced phosphorylation and subsequent Ca2+-dependent secretion in digitonin-treated cells.

     
  7. 7.

    Phorbol ester-induced secretion could be specifically inhibited by trypsin. The experiments indicate that protein kinase C modulates but is not necessary for Ca2+-dependent secretion.

     

Key words

exocytosis catecholamine adrenal chromaffin cell Ca2+ protein kinase digitonin 

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Copyright information

© Plenum Publishing Corporation 1988

Authors and Affiliations

  • Ronald W. Holz
    • 1
  1. 1.Department of PharmacologyUniversity of MichiganAnn ArborUSA

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