Cellular and Molecular Neurobiology

, Volume 5, Issue 3, pp 211–222 | Cite as

Quantitative autoradiographic characterization of receptors for angiotensin II and other neuropeptides in individual brain nuclei and peripheral tissues from single rats

  • Anita Israel
  • Laura M. Plunkett
  • Juan M. Saavedra
Review and Commentary


  1. 1.

    Autoradiographic techniques coupled with computerized microdensitometry and comparison with125I standards were used to characterize and quantitate receptors for neuropeptides in rat brain and adrenal and pituitary glands.

  2. 2.

    These techniques are rapidly performed, anatomically precise, and more sensitive than membrane binding techniques. They permit the determination of complete saturation curves and Scatchard analysis in discrete nuclei of the rat brain and in single rat pituitary and adrenal glands.

  3. 3.

    Angiotensin II (AII) receptors were quantitated after incubation of 16-µm tissue sections with the AII agonist125I-[Sar1]-AII.

  4. 4.

    High-affinity, high-density AII receptors were present in the organon subfornicalis, organon vasculosum laminae terminalis and nuclei triangularis septalis, suprachiasmatis, and paraventricularis of the rat and in rat adrenal capsule-zona glomerulosa area, adrenal medulla, and anterior pituitary.

  5. 5.

    These techniques could be used for precise localization and quantitation of other neuropeptide receptors in single rat brain nuclei, after optimizing the assay conditions and provided that suitable125I ligands are available.


Key words

quantitative autoradiography receptor autoradiography neuropeptide receptors angiotensin II receptors receptor quantitation in brain nuclei 


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  1. De Souza, E. B., Perrin, M. H., Insel, T. R., Rivier, J., Vale, W. W., and Kuhar, M. J. (1984). Corticotropinreleasing factor receptors in rat forebrain: Autoradiographic identification.Science 2241449–1451.Google Scholar
  2. Dufy-Barbe, L., Rodriguez, F., Arsaut, J., Verrier, D., and Vincent, J. D. (1982). Angiotensin stimulates prolactin release in the rhesus monkey.Neuroendocrinology 35242–247.Google Scholar
  3. Fitzsimons, J. T. (1980). Angiotensin stimulation of the central nervous system.Rev. Physiol. Biochem. Pharmacol. 87117–167.Google Scholar
  4. Goochee, C., Rasband, W., and Sokoloff, L. (1980). Computerized densitometry and color coding of [14C]deoxyglucose autoradiograms.Ann. Neurol. 7359–370.Google Scholar
  5. Hauger, R. L., Aguilera, G., Baukal, A. J., and Catt, K. J. (1982). Characterization of angiotensin II receptors in the anterior pituitary gland.Mol. Cell. Endocrinol. 25203–212.Google Scholar
  6. Helke, C. J., Shults, C. W., Chase, T. N., and O'Donohue, T. L. (1984). Autoradiographic localization of substance P receptors in rat medulla: Effect of vagotomy and nodose ganglionectomy.Neuroscience 12215–223.Google Scholar
  7. Israel, A., Correa, F. M. A., Niwa, M., and Saavedra, J. M. (1984a). Quantitative determination of angiotensin II binding sites in rat brain and pituitary gland by autoradiography.Brain Res. 322341–345.Google Scholar
  8. Israel, A., Correa, F. M. A., Niwa, M., and Saavedra, J. M. (1984b). Quantitative measurement of angiotensin II (AII) receptors in discrete regions of rat brain, pituitary and adrenal gland by autoradiography.Clin. Exp. Hyper.- Theory Pract. A6(10, 11):1761–1764.Google Scholar
  9. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193265–275.Google Scholar
  10. Mendelsohn, F. A. O., Aguilera, G., Saavedra, J. M., Quirion, R., and Catt, K. J. (1983). Characteristics and regulation of angiotensin II receptors in pituitary, circumventricular organs and kidney.Clin. Exp. Hyper. Theory Pract. A51081–1097.Google Scholar
  11. Mendelsohn, F. A. O., Quirion, R., Saavedra, J. M., Aguilera, G., and Catt, K. J. (1984). Autoradiographic localization of angiotensin II receptors in rat brain.Proc. Natl. Acad. Sci. USA 811575–1579.Google Scholar
  12. Mukherjee, A., Kulkarni, P., McCann, S. M., and Negro-Vilar, A. (1982). Evidence for the presence and characterization of angiotensin II receptors in rat anterior pituitary membranes.Endocrinology 110665–667.Google Scholar
  13. Munson, P. J. (1983). Ligand: A computerized analysis of ligand binding data. InMethods in Enzymology, Vol. 92. Immunochemical Techniques. Part E (J. J. Langone and H. Van Vunakis, Eds.), Academic Press, New York, pp. 543–576.Google Scholar
  14. Palacios, J. M., Niehoff, D. L., and Kuhar, M. J. (1981). Receptor autoradiography with tritium-sensitive film: Potential for computerized densitometry.Neurosci. Lett. 25101–105.Google Scholar
  15. Phillips, M. I. (1978). Angiotensin in the brain.Neuroendocrinology 25354–377.Google Scholar
  16. Rainbow, T. C., Bleisch, W. V., Biegon, A., and McEwen, B. S. (1982). Quantitative densitometry of neurotransmitter receptors.J. Neurosci. Methods 5127–138.Google Scholar
  17. Rainbow, T. C., Parsons, B., and Wolfe, B. B. (1984). Quantitative autoradiography ofβ 1- andβ 2-adrenergic receptors in rat brain.Proc. Natl. Acad. Sci. USA 811585–1589.Google Scholar
  18. Scott Young, W., III, and Kuhar, M. J. (1979). A new method for receptor autoradiography: [3H]Opioid receptors in rat brain.Brain Res. 179255–270.Google Scholar
  19. Sirett, N. E., McLean, A. S., Bray, J. J., and Hubbard, J. I. (1977). Distribution of angiotensin II receptors in rat brain.Brain Res. 122299–312.Google Scholar
  20. Steele, M. K., Negro-Vilar, A., and McCann, S. M. (1981). Effect of angiotensin II on in vivo and in vitro release of anterior pituitary hormones in the female rat.Endocrinology 109893–899.Google Scholar
  21. Tran, V. T., Uhl, G. R., Perry, D. C., Manning, D. C., Vale, W. W., Perrin, M. H., Rivier, J. E., Martin, J. B., and Snyder, S. H. (1984). Autoradiographic localization of somatostatin receptors in rat brain.Eur. J. Pharmacol. 101307–309.Google Scholar
  22. Unnerstall, J. R., Niehoff, D. L., Kuhar, M. J., and Palacios, J. M. (1982). Quantitative receptor autoradiography using [3H]Ultrofilm: Application to multiple benzodiazepine receptors.J. Neurosci. Methods 659–73.Google Scholar
  23. Zarbin, M. A., Innis, R. B., Wamsley, J. K., Snyder, S. H., and Kuhar, M. J. (1983). Autoradiographic localization of cholecystokinin receptors in rodent brain.J. Neurosci. 3877–906.Google Scholar

Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Anita Israel
    • 1
    • 2
  • Laura M. Plunkett
    • 1
  • Juan M. Saavedra
    • 1
  1. 1.Section on Clinical Pharmacology, Laboratory of Clinical ScienceNational Institute of Mental HealthBethesdaUSA
  2. 2.Guest Researcher from School of PharmacyCentral UniversityCaracasVenezuela

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