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Chromosome Research

, Volume 3, Issue 6, pp 351–360 | Cite as

Mapping functional domains of the Polycomb protein ofDrosophila melanogaster

  • Axel Franke
  • Sabine Messmer
  • Renato ParoEmail author
Article

Abstract

InDrosophila the Polycomb group (Pc-G) proteins are responsible for the stable and heritable silencing of genes. The Pc-G apparently uses heterochromatin-like mechanisms to transcriptionally inactivate developmental regulators such as the homeotic genes. The Polycomb (Pc) protein is part of a large multimeric complex composed of other members of the Pc-G. We have identified functionally relevant domains of the Pc protein by sequencing differentPc alleles. Additionally, using a Pc-βgal fusion protein with deleted internal histidine repeats, we found that this mutant protein cannot bind to four particular target loci, but otherwise does not change the remaining overall binding pattern. We show that, in contrast to the dotted subnuclear localization of the wild-type protein, the nuclear distribution of mutant proteins becomes homogeneous. Surprisingly, inPc mutants the polyhomeotic protein, another member of the Pc-G, is also redistributed in the nucleus. Our results indicate that the appropriate subnuclear localization of the two proteins is critical for the silencing function of the Pc-G complex.

Key words

chromatin Drosophila melanogaster Polycomb silencing 

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Copyright information

© Rapid Communications of Oxford Ltd. 1995

Authors and Affiliations

  1. 1.the Department of Biological SciencesStanford UniversityStanfordUSA
  2. 2.DIANOVA-immunotechHamburgGermany
  3. 3.Zentrum für Molekulare BiologieUniversität HeidelbergHeidelbergGermany

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