Journal of comparative physiology

, Volume 134, Issue 4, pp 345–350 | Cite as

Physiological and biochemical adaptations to training inRana pipiens

  • John W. Cummings
Article

Summary

FifteenRana pipiens were trained on a treadmill thrice weekly for 6.5 weeks to assess the effects of training on an animal that supports activity primarily through anaerobiosis. Endurance for activity increased 35% in these frogs as a result of training (P=0.006, Fig. 1). This increased performance was not due to enhanced anaerobiosis. Total lactate produced during exercise did not differ significantly for the trained or untrained animals in either gastrocnemius muscle (2.77±0.21 and 2.82±0.13 mg/g, respectively) or whole body (1.32±0.10 and 1.47±0.06 mg/g, respectively). Glycogen depletion also did not differ between the two groups (Fig. 2c). The primary response to training appeared to involve augmentation of aerobic metabolism, a response similar to that reported for mammals. Gastrocnemius muscles of trained frogs underwent a 38% increase over those of untrained individuals in the maximum activity of citrate synthase (14.5±1.0 and 10.5±0.9 μmoles/(g wet wt·min);P=0.008). This enzyme was also positively correlated with the level of maximum performance for all animals tested (r=0.61,P<0.01) and with the degree of improvement in the trained animals (r=0.72,P<0.05). In addition to an increased aerobic capacity, the trained animals demonstrated a greater removal of lactate from the muscle 15 min after fatigue (Fig. 2b).

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References

  1. Alp, P.R., Newsholme, E.A., Zammit, V.A.: Activities of citrate synthase and NAD+-linked isocitrate dehydrogenase in muscle from vertebrates and invertebrates. Biochem. J.154, 689–700 (1976)Google Scholar
  2. Andersen, P., Henriksson, J.: Capillary supply of the quadriceps femoris muscle of man: adaptive response to exercise. J. Physiol.270, 677–690 (1977)Google Scholar
  3. Baldwin, K.M., Klinkerfuss, G.H., Terjung, R.L., Molé, P.A., Holloszy, J.O.: Respiratory capacity of white, red and intermediate muscle: adaptive response to exercise. Am. J. Physiol.222, 373–378 (1972)Google Scholar
  4. Bendall, J.R., Taylor, A.A.: The Meyerhof quotient and the synthesis of glycogen from lactate in frog and rabbit muscle. A reinvestigation. Biochem. J.118, 887–893 (1970)Google Scholar
  5. Bennett, A.F., Gleeson, T.T.: Activity metabolism in the lizardSceloporus occidentalis. Physiol. Zool.49, 65–79 (1976)Google Scholar
  6. Bennett, A.F., Licht, P.: Anaerobic metabolism during activity in lizards. J. Comp. Physiol.81, 277–288 (1972)Google Scholar
  7. Bennett, A.F., Licht, P.: Anaerobic metabolism during activity in amphibians. Comp. Biochem. Physiol.48A, 319–327 (1974)Google Scholar
  8. Brace, R.A.: Fitting straight lines to experimental data. Am. J. Physiol.233, R94-R99 (1977)Google Scholar
  9. Carey, C.: Thermal physiology and energetics of boreal toads,Bufo boreas boreas. Ph.D. Dissertation, University of Michigan (1976)Google Scholar
  10. Costill, D.L., Daniels, J., Evans, W., Fink, W., Krahenbuhl, G., Saltin, B.: Skeletal muscle enzymes and fiber composition in male and female track athletes. J. Appl. Physiol.40, 149–154 (1976)Google Scholar
  11. Crabtree, B., Higgins, S.J., Newsholme, E.A.: The activities of pyruvate carboxylase, phosphoenolpyruvate carboxylase and fructose diphosphatase in muscles from vertebrates and invertebrates. Biochem. J.130, 391–396 (1972)Google Scholar
  12. Dohm, G.L., Huston, R.L., Askew, E.W., Fleshood, H.L.: Effects of exercise, training and diet on muscle citric acid cycle enzyme activity. Can. J. Biochem.51, 849–854 (1973)Google Scholar
  13. Gleeson, T.T.: The effects of training and captivity on the metabolic capacity of the lizardSceloporus occidentalis. J. Comp. Physiol.129, 123–128 (1979)Google Scholar
  14. Gollnick, P.D., King, D.W.: Effect of exercise and training on mitochondria of rat skeletal muscle. Am. J. Physiol.216, 1502–1509 (1969)Google Scholar
  15. Fitts, R.H., Booth, F.W., Winder, W.W., Holloszy, J.O.: Skeletal muscle respiratory capacity, endurance, and glycogen utilization. Am. J. Physiol.228, 1029–1033 (1975)Google Scholar
  16. Hammond, B.R., Hickman, C.P.: The effect of physical conditioning on the metabolism of lactate, phosphate, and glucose in rainbow trout,Salmo gairdneri. J. Fish. Res. Bd. Canada23, 65–83 (1966)Google Scholar
  17. Henriksson, J., Reitman, J.S.: Time course of changes in human skeletal muscle succinate dehydrogenase and cytochrome oxidase activities and maximal oxygen uptake with physical activity and inactivity. Acta Physiol. Scand.99, 91–97 (1977)Google Scholar
  18. Hermansen, L.: Lactate production during exercise. In: Muscle metabolism during exercise. Pernow, B., Saltin, B. (eds.), pp. 401–407. New York: Plenum 1971Google Scholar
  19. Hermansen, L., Vaage, O.: Lactate disappearance and glycogen synthesis in human muscle after maximal exercise. Am. J. Physiol.233, E422-E429 (1977)Google Scholar
  20. Holloszy, J.O.: Biochemical adaptations in muscle. Effects of exercise on mitochondrial oxygen uptake and respiratory enzyme activity in skeletal muscle. J. Biol. Chem.242, 2278–2282 (1967)Google Scholar
  21. Holloszy, J.O., Booth, F.W.: Biochemical adaptations to endurance exercise in muscle. Ann. Rev. Physiol.38, 273–291 (1976)Google Scholar
  22. Holloszy, J.O., Oscai, L.B., Don, I.J., Molé, P.A.: Mitochondrial citric acid cycle and related enzymes: adaptive response to exercise. Biochem. Biophys. Res. Commun.40, 1368–1373 (1970)Google Scholar
  23. Hutchison, V.H., Turney, L.D.: Glucose and lactate concentrations during activity in the leopard frog,Rana pipiens. J. Comp. Physiol.99, 287–295 (1975)Google Scholar
  24. Keppler, D., Decker, K.: Glycogen determination with amyloglucosidase. In: Methods of enzymatic analysis. Bergmeyer, H.U., (ed.), pp. 1127–1131. New York: Academic Press 1974Google Scholar
  25. Krebs, H.A., Woodford, M.: Fructose 1,6-diphosphatase in striated muscle. Biochem. J.94, 436–445 (1965)Google Scholar
  26. Maxwell, L.C., Barclay, J.K., Mohrman, D.E., Faulkner, J.A.: Physiological characteristics of skeletal muscles of dogs and cats. Am. J. Physiol.233, C14-C18 (1977)Google Scholar
  27. Molé, P.A., Oscai, L.B., Holloszy, J.O.: Adaptation of muscle to exercise: increase in levels of palmityl CoA synthetase, carnitine palmityltransferase, and palmityl CoA dehydrogenase, and in the capacity to oxidize fatty acids. J. Clin. Invest.50, 2323–2330 (1971)Google Scholar
  28. Opie, L.H., Newsholme, E.A.: The activities of fructose 1,6-diphosphatase, phosphofructokinase, phosphoenolpyruvate carboxykinase in white and red muscle. Biochem. J.103, 391–399 (1967)Google Scholar
  29. Putnam, R.W.: Relationship between fatigue and changes of lactate and glycogen in muscles of anuran amphibians. Am. Zool.17, 893 (1977)Google Scholar
  30. Srere, P.A.: Citrate synthase. In: Methods in enzymology, Vol. 13. Lowenstein, J.W. (ed.), pp. 3–11. New York: Academic Press 1969Google Scholar
  31. Winder, W.W., Baldwin, K.M., Holloszy, J.O.: Enzymes involved in ketone utilization in different types of muscle: adaptation to exercise. Eur. J. Biochem.47, 461–467 (1974)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • John W. Cummings
    • 1
  1. 1.Division of Biological SciencesThe University of MichiganAnn ArborUSA

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