Biology and Fertility of Soils

, Volume 7, Issue 3, pp 213–218 | Cite as

Competitiveness and persistence of strains ofRhizobium phaseoli introduced into a Moroccan sandy soil

  • A. Hilali
  • J. Aurag
  • J. A. E. Molina
  • E. L. Schmidt


Sixteen strains ofRhizobium phaseoli were isolated from the Loukkos sandy soil and were compared with four selected strains ofR. phaseoli (CIAT 676, CIAT 57, Olivia, Viking 1) for their N2-fixing ability and for their serological affinities by means of the fluorescent antibody technique. Two indigenous strains were rated as highly effective, six as moderately effective, and eight were ineffective. Viking 1 was ranked as highly effective while the other inoculum strains were moderately effective. None of the 4 inoculum strains cross-reacted with the 16 indigenous strains. The indigenous strains were grouped into only two serogroups but showed a high degree of heterogeneity in regard to the strength of the immunofluorescence response. The inoculum strains outcompeted but did not eliminate the resident population for nodule occupancy on two French bean cultivars (Royalnel and Fetiche). The competitiveness, however, differed between inoculum strains. It was influenced by the host cultivar and by the host cultivar growth stage. Viking 1 was consistent in its high competitive ability whether it was inoculated singly or in a mixture with the other strains. It showed high persistence and formed more than 50% of the nodules 1 year after it was introduced. CIAT 57, Olivia, and CIAT 676 were mediocre in their persistence.

Key words

Rhizobium phaseoli Competition Persistence Strain-cultivar interaction Cultivar interaction Growth chamber 


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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • A. Hilali
    • 1
  • J. Aurag
    • 1
  • J. A. E. Molina
    • 2
  • E. L. Schmidt
    • 2
  1. 1.Institut Agronomique et Vétérinaire Hassan IIRabat-Agdal, RabatMorocco
  2. 2.Department of Soil ScienceUniversity of MinnesotaSt. PaulUSA

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