Histochemistry

, Volume 82, Issue 2, pp 141–148 | Cite as

Localization of cathepsin D in rat liver

Immunocytochemical study using post-embedding immunoenzyme and protein A-gold techniques
  • S. Yokota
  • H. Tsuji
  • K. Kato
Article
Accepted:

Summary

Light and electron microscopic localization of cathepsin D in rat liver was investigated by post-embedding immunoenzyme and protein A-gold techniques. By light microscopy, cytoplasmic granules of parenchymal cells and Kupffer cells were stained for cathepsin D. Weak staining was also noted in sinusoidal endothelial cells. In the parenchymal cells many of positive granules located around bile canaliculi. In the Kupffer cells and the endothelial cells, diffuse staining was noted in the cytoplasm in addition to granular staining. By electron microscopy, gold particles representing the antigenic sites for cathepsin D were seen in typical secondary lysosomes and some multivesicular bodies of the parenchymal cells and Kupffer cells. The lysosomes of the endothelial cells and fat-storing cells were weakly labeled. Quantitative analysis of the labeling density in the lysosomes of these three types of cells demonstrated that the lysosomes of parenchymal cells and Kupffer cells are main containers of cathepsin D in rat liver. The results suggest that cathepsin D functions in the intracellular digestive system of parenchymal cells and Kupffer cells but not so much in that of the endothelial cells.

Keywords

Endothelial Cell Gold Particle Parenchymal Cell Kupffer Cell Antigenic Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • S. Yokota
    • 1
  • H. Tsuji
    • 2
  • K. Kato
    • 2
  1. 1.Department of AnatomyYamanashi Medical SchoolYamanashiJapan
  2. 2.Department of Physiological Chemistry, Faculty of Pharmaceutical ScienceKyushu UniversityFukuokaJapan

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