Nitrate induces enzymes of the mannitol cycle and suppresses versicolorin synthesis in Aspergillus parasiticus Abstract
Addition of sodium nitrate to growing cultures of
Aspergillus parasiticus (ATCC 36537) induces the synthesis of enzymes involved in nitrate assimilation (NO reductase), of enzymes in the pentose pathway (glucose-6-phosphate dehydrogenase), and of enzymes in the mannitol cycle (mannitol- and mannitol-1-phosphate dehydrogenases). Addition of NO 3 − also causes a dose-dependent suppression of synthesis of the polyketide secondary metabolite, versicolorin A. We suggest that in the presence of NO 3 − plus peptone, the cytoplasmic NADPH/NADP ratio may be elevated, resulting in increased conversion of malonyl coenzyme A to fatty acid rather than to polyketide. 3 − Key words Aspergillus parasiticus ATCC 36537 enzyme induction mannitol dehydrogenase mannitol-1-phosphate dehydrogenase NADPH/NADP ratio nitrate reductase References
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