Pflügers Archiv

, Volume 429, Issue 4, pp 578–586 | Cite as

Role of protein kinase C in the regulation of inositol phosphate production and Ca2+ mobilization evoked by ATP and acetylcholine in rat lacrimal acini

  • Jesper Gromada
  • Tino D. Jørgensen
  • Steen Dissing
Original Article Molecular and Cellular Physiology

Abstract

Stimulation of rat lacrimal acinar cells with ATP and acetylcholine (ACh) induced a rapid accumulation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] and its degradation products, resulting in an initial release of Ca2+ from intracellular stores. However, after pretreating the acini with U73122 no increase in the intracellular free Ca2+ concentration ([Ca2+]i) or Ins(1,4,5)P3 production was observed. A short pre-treatment with the phorbol ester 4-β-phorbol-12-β-myristate-13-α-acetate (PMA) significantly attenuated the ATP- and ACh-induced increase in [Ca2+]i and over- all inositol phosphate production. In contrast, staurosporine enhanced Ins(1,4,5)P3 and inositol 1,3,4-trisphosphate [Ins(1,3,4)P3] production and [Ca2+]i above control values in ATP- and ACh-stimulated cells. Stimulation of phospholipase C by iono-mycin-evoked changes in [Ca2+]i were unaltered by pretreatment with staurosporine and PMA. The data show that a change in protein kinase C activity during cell stimulation affects the inositol phosphate metabolism and thereby the cellular Ca2+ signalling processes in lacrimal acinar cells.

Key words

Lacrimal acinar cell ATP Protein kinase C Inositol phosphates Ca2+ mobilization Acetylcholine U73122 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Jesper Gromada
    • 1
  • Tino D. Jørgensen
    • 1
  • Steen Dissing
    • 1
  1. 1.Department of Medical Physiology, The Panum InstituteUniversity of CopenhagenCopenhagen NDenmark

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