Human Genetics

, Volume 80, Issue 2, pp 111–116

Sorting of chromosomes by magnetic separation

  • Gertrud Dudin
  • Ernst W. Steegmayer
  • Peter Vogt
  • Haide Schnitzer
  • Eduardo Diaz
  • Kathryn E. Howell
  • Thomas Cremer
  • Christoph Cremer
Original Investigations

DOI: 10.1007/BF00702851

Cite this article as:
Dudin, G., Steegmayer, E.W., Vogt, P. et al. Hum Genet (1988) 80: 111. doi:10.1007/BF00702851
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Summary

Chromosomes were isolated from Chinese hamster x human hybrid cell lines containing four and nine human chromosomes. Human genomic DNA was biotinylated by nick translation and used to label the human chromosomes by in situ hybridization in suspension. Streptavidin was covalently coupled to the surface of magnetic beads and these were incubated with the hybridized chromosomes. The human chromosomes were bound to the magnetic beads through the strong biotin-streptavidin complex and then rapidly separated from nonlabeled Chinese hamster chromosomes by a simple permanent magnet. The hybridization was visualized by additional binding of avidin-FITC (fluorescein) to the unoccupied biotinylated human DNA bound to the human chromosomes. After magnetic separation, up to 98% of the individual chromosomes attached to magnetic beads were classified as human chromosomes by fluorescence microscopy.

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Gertrud Dudin
    • 1
  • Ernst W. Steegmayer
    • 2
  • Peter Vogt
    • 2
  • Haide Schnitzer
    • 1
  • Eduardo Diaz
    • 1
  • Kathryn E. Howell
    • 3
  • Thomas Cremer
    • 2
  • Christoph Cremer
    • 1
  1. 1.Institut für Angewandte Physik I der UniversitätHeidelbergGermany
  2. 2.Institut für Humangenetik und Anthropologie der UniversitätHeidelbergFedoral Republic of Germany
  3. 3.European Molecular Biology Laboratory (EMBL)HeidelbergGermany

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