Acta Neuropathologica

, Volume 45, Issue 3, pp 187–194 | Cite as

Cell origin of human adenovirus type 12-induced subcutaneous tumor in Syrian hamsters

  • T. Nakajima
  • N. Mukai
Original Works


Single subcutaneous inoculation of human adenovirus type 12 (Ad. 12), 0.05–0.1 ml of 108.0 TCID50 HEK cells/0.1 ml, was made on the back of 0-day-old hamsters. In 21 of 25 hamsters (84.0%), multiple solid tumors developed close to the inoculation site within 3 months. No control hamsters developed tumors. Tumor histopathology revealed the characteristic Homer Wright rosettes of neuroblastoma. Ad. 12-specific tumor antigens were demonstable in both the primary and the cultured tumor cells by the hnmunofluorescein technique. Histochemical demonstration of cholinesterase and NADH oxidoreductase gave rise to a predominantly positive intracytoplasmic granule within the tumor cells. Electron microscopy showed remarkably iniform cell morphology: small, undifferentiated neuroblastic cells with poorly developed intracytoplasmic organelles; many possessed characteristic solitary cilia in a 9+0 tubules pattern. Intercellular junctions were poorly developed. Search for an incipient tumor cell aggregate by means of immunofluorescein T-antigen detection was carried out through a 240-h period following Ad. 12 inoculation. A sequential study in parallel with electron microscopic examination of the normal subcutaneous tissue proved that neuroblastic cells closely associated with the muscle spindle anlage could preferentially become the most sensitive target for Ad. 12 tumorigenesis.

Key words

Human adenovirus 12 Hamsters Subcutaneous neuroblastomatous tumor T-antigens Electron microscopy 


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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • T. Nakajima
    • 1
  • N. Mukai
    • 1
    • 2
  1. 1.Wesley C. Bowers Laboratory of Pharmacology and Experimental PathologyEye Research Institute of Retina FoundationBostonUSA
  2. 2.Department of Ophthalmology (Neuropathology)Massachusetts Eye and Ear Infirmary, and Harvard Medical SchoolBostonUSA

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