Immunogenetics

, Volume 31, Issue 1, pp 29–36

The nucleotide sequence of the bovine MHC class II alpha genes:DRA, DQA, andDYA

  • Jan J. van der Poel
  • Martien A. M. Groenen
  • Rosilde J. M. Dijkhof
  • Dirk Ruyter
  • Marius J. Giphart
Article

Abstract

The nucleotide sequence of the exons 2, 3, and 4, and parts of the intervening sequences of aBoLA-DRA and-DQA gene and one other class IIBoLA-A gene have been determined. The structure of theBoLA-DRA and-DQA gene was found to be very similar to that of the corresponding human HLA class II genes. An analysis of the structure of the other class IIBoLA-A gene showed that thisA gene was clearly very different from both the humanA genes and the bovineDRA andDQA genes. The results indicate that this other type of class IIA gene probably represents the class II gene that has already been identified in restriction fragment length polymorphism (RFLP) studies asBoLA-DYA. Since no clear homologue of this presumedBoLA-DYA gene was found among the human HLA class II genes, these results indicate that, at least as far as theA genes are concerned, a distinct class II gene is present in cattle.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Andersson, L. and Rask, L.: Characterization of the MHC class II region in cattle. The number ofDQ genes varies between haplotypes.Immunogenetics 27: 110–120, 1988Google Scholar
  2. Andersson, L., Bohme, J., Rask, L., and Peterson, P. A.: Genomic hybridization of bovine class II major histocompatibility genes. 1. Extensive polymorphism of DQ alpha and DQ beta genes.Anim Genet 17: 95–112, 1986aGoogle Scholar
  3. Andersson, L., Bohme, J., Peterson, P. A., and Rask, L.: Genomic hybridization of bovine class II genes. 2. Polymorphism of DR genes and linkage disequilibrium in the DQ-DR region.Anim Genet 17: 295–304, 1986bGoogle Scholar
  4. Andersson, L., Lunden, A., Sigurdardottir, S., Davies, C. J., and Rask, L.: Linkage relationships in the bovine MHC region. High recombination frequency between class II subregions.Immunogenetics 27: 273–280, 1988Google Scholar
  5. Auffray, C. and Strominger, J. L.: Molecular genetics of the human major histocompatibility complex.Adv Hum Genet 15: 197–247, 1986Google Scholar
  6. Auffray, C., Lillie, J. W., Arnot, D., Grossberger, D., Kappes, D., and Strominger, J. L.: Isotypic and allotypic variation of human class II histocompatibility α-chain genes.Nature 308: 327–333, 1984Google Scholar
  7. Auffray, C., Lillie, J. W., Korman, A. J., Boss, J. M., Frechin, N., Guillemot, F., Cooper, J., Mulligan, P. C., and Strominger, J. L.: Structure and expression ofHLA-DQ α and-DX α genes: interallelic alternate splicing of theHLA-DQ α gene and functional splicing of theHLA-DX α gene using a retroviral vector.Immunogenetics 26: 63–73, 1987Google Scholar
  8. Birnboim, H. C. and Doly, J.: A rapid alkaline extraction procedure for screening plasmid DNA.Nucleic Acids Res 7: 1513–1523, 1979Google Scholar
  9. Chang, H. C., Moriuchi, T., and Silver, J.: The heavy chain of the human B cell alloantigen HLA-DS, has a variable N terminal region and a constant immunoglobulin-like region.Nature 305: 813–815, 1983Google Scholar
  10. Das, H. K., Lawrence, S. K., and Weissman, S. M.: Structure and nucleotide sequence of the heavy chain gene of HLA-DR.Proc Natl Acad Sci USA 80: 3542–3547, 1983Google Scholar
  11. Groenen, M. A. M., van der Poel, J. J., Dijkhof, R. J. M., and Giphart, M. J.: Cloning of the bovine major histocompatibility complex class II genes.Anim Genet 20: 267–268, 1989Google Scholar
  12. Groenen, M. A. M., van der Poel, J. J., Dijk, R. J. M., and Giphart, M. J.: The nucleotide sequence of the bovine MHC class IIDQB andDRB genes.Immunogenetics 31: 37–44, 1990Google Scholar
  13. Kappes, D. and Strominger, J. L.: Human class II major histocompatibility complex genes and proteins.Ann Rev Biochem 57: 991–1028, 1988Google Scholar
  14. Kaufman, J. F., Auffray, C., Korman, A. J., Shackelford, D. A., and Strominger, J. L.: The class II molecules of the human and murine major histocompatibility complex.Cell 36: 1–13, 1984Google Scholar
  15. Klein, J.: The major histocompatibility complex of the mouse.Science 203: 516–521, 1979Google Scholar
  16. Klein, J.Natural History of the Major Histocompatibility Complex, Wiley, New York, 1986Google Scholar
  17. Larhammar, D., Gustafson, K., Claesson, L., Bill, P., Wiman, K., Schenning, L., Sundelin, J., Widmark, E., Peterson, P. A., and Rask, L.: Alpha chain of HLA-DR transplantation antigens is a member of the same protein super family as the immunoglobulins.Cell 30: 153–161, 1982Google Scholar
  18. Lawrence, S. K., Das, H. K., Pan, J., and Weissman, S. M.: The genomic organization and nucleotide sequence of the HLA-SB(DP) alpha gene.Nucleic Acids Res 13: 7515–7528, 1985Google Scholar
  19. LeGuern, C., Weissman, J. D., Marche, P. N., Jouvin-Marche, E., Laverrière, A., Bagnato, M. R., and Kindt, T. J.: Sequence determination of a transcribed rabbit class II gene with homology toHLA-DQ α.Immunogenetics 25: 104–109, 1987Google Scholar
  20. Mount, S. M.: A catalogue of splice junction sequences.Nucleic Acid Res 10: 459–472, 1982Google Scholar
  21. Muggli-Cockett, N. E. and Stone, R. T.: Identification of genetic variation in the bovine major histocompatibility complex DRβ-like genes using sequenced bovine genomic probes.Anim Genet 19: 213–225, 1988Google Scholar
  22. Schenning, L., Larhammer, D., Bill, P., Wiman, K., Jonsson, A., Rask, L., and Peterson, P. A.: Both α and β chains of HLA-DC class II histocompatibility antigens display extensive polymorphism in their amino-terminal domains.EMBO J 3: 447–452, 1984Google Scholar
  23. Tabor, S. and Richardson, C. C.: DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.Proc Natl Acad Sci USA 84: 4767–4771, 1987Google Scholar
  24. Trowsdale, J. and Kelly, A.: The human HLA class II α chain DZα is distinct from genes in the DP, DQ subregions.EMBO J 4: 2231–2237, 1985Google Scholar
  25. Usinger, W. R., Curie-Cohen, M., and Stone, W. H.: Lymphocyte defined loci in cattle.Science 196: 1017–1018, 1977Google Scholar
  26. Verhoeven, A. J. M., Roos, M. H., and Ploegh, H. L.: A feline class II α gene with striking similarity to theHLA-DPA pseudogene.Immunogenetics 28: 406–411, 1988Google Scholar
  27. White, J. J., Arnheim, N., and Ehrlich, H. A.: The Polymerase chain reaction.Trends in Genet 5: 185–189, 1989Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Jan J. van der Poel
    • 1
  • Martien A. M. Groenen
    • 1
  • Rosilde J. M. Dijkhof
    • 1
  • Dirk Ruyter
    • 1
  • Marius J. Giphart
    • 1
    • 2
  1. 1.Department of Animal BreedingAgricultural University WageningenWageningenThe Netherlands
  2. 2.Department of Immunohaematology and BloodbankUniversity HospitalLeidenThe Netherlands

Personalised recommendations