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Glycoconjugate Journal

, Volume 13, Issue 5, pp 839–847 | Cite as

MUC5AC, but not MUC2, is a prominent mucin in respiratory secretions

  • Hans W. Hovenberg
  • Julia R. Davies
  • Annkatrin Herrmann
  • Carl-Johan Lindén
  • Ingemar Carlstedt
Papers

Abstract

Airway mucus was collected from healthy and chronic bronchitic subjects. The chronic bronchitic sputum was separated into gel and sol phase by centrifugation and mucins were isolated using isopycnic density-gradient centrfugation in CsCl. The presence of the MUC5AC and MUC2 mucins was investigated with antisera raised against synthetic peptides with sequences from the respective apoproteins. The gel and sol phase of chronic bronchitic sputum as well as healthy respiratory secretions were shown to contain MUC5AC whereas the MUC2 mucin could not be detected. Rate-zonal centrifugation showed that the MUC5AC mucin was large, polydisperse in size and that reduction yielded subunits. Ion-exchange HPLC revealed the presence of two subunit populations in all secretions, the MUC5AC subunits always being the more acidic. MUC5AC is thus the first large, subunit-based, gel-forming respiratory mucin identified and this glycoprotein is biochemically distinct from at least one other population of large, gel-forming mucins also composed of subunits but lacking a genetic identity.

Keywords

mucin trachea secretion MUC5AC MUC2 

Abbreviations

CHAPS

3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate

CF

cystic fibrosis

DFP

diisopropylphosphofluoridate

DTT

dithiothreitol

EDTA

ethylenedinitrilotetraacetic acid

NEM

N-ethylmaleimide

PAS

periodic acid/Schiffs

PMSF

phenylmethylsulphonyl fluoride

Tris

Tris(hydroxymethyl)aminomethane

VNTR

variable number of tandem repeats

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Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • Hans W. Hovenberg
    • 1
  • Julia R. Davies
    • 1
  • Annkatrin Herrmann
    • 1
  • Carl-Johan Lindén
    • 2
  • Ingemar Carlstedt
    • 1
  1. 1.Department of Cell and Molecular Biology, Section for Molecular PathogenesisLund UniversityLundSweden
  2. 2.Department of Lung MedicineLund University HospitalLundSweden

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