Molecular and General Genetics MGG

, Volume 146, Issue 3, pp 247–251 | Cite as

The location of the feedback-specific region within thePyrimidine-3 locus ofNeurospora crassa

  • Andrew J. Makoff
  • Alan Radford


Thepyrimidine-3 locus ofNeurospora crassa specifies two enzyme activities, pyrimidine-specific carbamyl phosphate synthetase (CPSpyr) and aspartate transcarbamylase (ATC). ATC is translationally distal. CPSpyr, but not ATC, is subject to feedback inhibition by uridine triphosphate (UTP). To investigate the location of the feedback-specific region within the locus, inhibition of a number ofpyr-3 alleles by UTP was investigated. All CPS+ ATC- polar alleles, revertants of CPS- ATC- polar alleles, and 5-fluorouracil-resistant mutants had normal UTP response. The location of the feedback-specific region is in or close to the CPS-specific region.


Enzyme Phosphate Enzyme Activity Aspartate Triphosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Andrew J. Makoff
    • 1
  • Alan Radford
    • 1
  1. 1.Department of GeneticsUniversity of LeedsLeedsU.K.

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