Summary
Electric field orientation of bacteriorhodopsin (BRp) in the form of purple membrane (PM) in synthetic polymer membranes made from poly(hydroxy-methyl methacrylate) or photo-crosslinkable propyleneoxide-based prepolymer was achieved by combining once-desalted and liophilized PM sample and ethylene glycol as a dilutant, which was detected by an increase in photoelectric current or membrane potential, respectively.
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References
D.Oesterhelt and W.Stoeckenius, Nature New Biol.,233, 149 (1971).
W.Stoeckenius, R.H.Lozier, and R.A.Bogomolni, Biochim. Biophys. Acta,505, 215 (1979).
e.g. P.Shieh, and L.Packer, Biochem.Biophys.Res.Commun.72, 603 (1976).
e.g. M.C.Block, K.J.Hellingwerf, and K. van Dam, FEBS Lett.,76, 45 (1977).
L.Ketszthelyie, Biochim.Biophys.Acta,598, 429 (1980).
L.D.Kahn, and Shu-Itu, Biopolymers,23, 707 (1984).
Y.Kimura, A.Ikegami, K.Ohno, S.Saigo, and Y.Takeuchi, Photochem. Photobiol.33, 435 (1981).
G.Todorov, S.Sokerov, and S.P.Stoylov, Biophys.J.,40, 1 (1982).
Y.Kimura, M.Fujiwara, and A.Ikegami, Biophys.J.,45, 615 (1984).
M.Eisenbach, C.Weissman, G.Tanny, and S.R.Caplan, FEBS Lett.,81, 1 (1977).
K.Sigh, R.Korenstein, H.Lebedeva, and S.R.Caplan, Biophys.J.,31, 393 (1980).
K.Nagy, Biochem.Biophys.Res.Comm.,81, 383 (1978).
G.Varo, Acta Biol.Acad.Sci.Hung.,132,301 (1981).
S.R.Caplan and G.Fisher, J. Membr. Sci.,16, 391 (1983).
A.A.Kononenko, E.P.Lukashev, A.V.Maximychev, S.K.Chamorovsky, A.B.Rubin, S.F.Timashev and L.N.Chekulaeva, Biochim.Biophys.Acta,850, 162 (1986)
Y.Nakamura, Y.Saito, Y.Obata, and S.Kunugi, J.Soc.Fib.Sci.Tech. Japan.41 173 (1985).
S.Kunugi, H.Kodama, H.Yamada, and Y.Nakamura,J.Soc.Fib.Sci.Tech. Japan.41, 355 (1985).
S.Kunugi, H.Yamada, T.Nakajima, and Y.Nakamura, Membrane(Maku),12, 101 (1987).
S.Kunugi, H.Yamada, Y.Nakamura, F.Tokunaga, and A.Tanaka, Polymer Bull.,18, 87 (1987)
S.Kunugi, T.Kusano, H.Yamada, and Y.Nakamura, Polymer Bull.,19, 417 (1988).
S.Fukui and A.Tanaka, Adv.Biochemical Engineering/Biotechnology,29, 1 (1985).
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We gratefully acknowledge the kind advice and helpful discussion of Professor Fumio Tokunaga and Dr. Tatsuro Iwasa (Tohoku University).
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Kunugi, S., Tatsukawa, K., Nakajima, T. et al. Orientation of bacteriorhodopsin in non-aqueous polymer membrane. Polymer Bulletin 21, 59–62 (1989). https://doi.org/10.1007/BF00700269
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DOI: https://doi.org/10.1007/BF00700269