A cell line derived from the urothelium lining the ureter of a 12-year-old girl was immortalised using a temperature-sensitive SV40 large T-antigen gene construct, and designated UROtsa. Following immortalisation, UROtsa cells expressed SV40 large T-antigen, but did not acquire characteristics of neoplastic transformation, including growth in soft agar or the development of tumours in nude mice. Metaphase spreads had a normal chromosomal appearance and number. UROtsa cells remained permissive for cell growth at 39°C, indicating that they did not retain temperature sensitivity. UROtsa provides an in vitro model of “normal” urothelium.
Key wordsNormal urothelium In vitro Immortalisation
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- 1.Bookland EA, Swaminathan S, Oyasu R, Gilchrist KW, Lindstrom M, Reznikoff CA (1992) Tumorigenic transformation and neoplastic progression of human uroepithelial cells after exposure in vitro to 4-aminobiphenyl or its metabolites. Cancer Res 52:1606Google Scholar
- 2.Christian BJ, Loretz LJ, Oberley TD, Reznikoff CA (1987) Characterization of human uroepithelial cells immortalized in vitro by simian virus 40. Cancer Res 47:6066Google Scholar
- 3.Hayflick L (1965) The limited in vitro lifespan of human diploid cell lines. Exp Cell Res 37:614Google Scholar
- 4.Jat PS, Sharp PA (1989) Cell lines established by a temperature-sensitive simian virus 40 large-T-antigen gene are growth restricted at the nonpermissive temperature. Mol Cell Biol 9:1672Google Scholar
- 5.Kao C, Wu S-Q, De Vries S, Reznikoff WS, Waldman FM, Reznikoff CA (1993) Carcinogen-induced amplification of SV40 DNA inserted at 9q12-21.1 associated with chromosome breakage, deletions, and translocations in human uroepithelial cell transformation in vitro. Genes Chromosom Cancer 8:155Google Scholar
- 6.Meisner LF, Wu S-Q, Christian BJ, Reznikoff CA (1988) Cytogenetic instability with balanced chromosome changes in an SV40 transformed human uroepithelial cell line. Cancer Res 48:3215Google Scholar
- 7.Pazzaglia S, Chen XR, Aamodt CB, Wu S-Q, Gilchrist KW, Oyasu R, Reznikoff CA, Ritter MA (1994) In vitro radiation-induced neoplastic progression of low-grade uroepithelial tumors. Radiat Res 138:86Google Scholar
- 8.Petzoldt JL, Leigh IM, Duffy PG, Masters JRW (1994) Culture and characterisation of human urothelium in vivo and in vitro. Urol Res 22:67Google Scholar
- 9.Pratt CI, Wu S-Q, Bhattacharya M, Kao C, Gilchrist KW, Reznikoff CA (1992) Chromosome losses in tumorigenic revertants of EJ/ras-expressing somatic cell hybrids. Cancer Genet Cytogenet 59:180Google Scholar
- 10.Reznikoff CA, Loretz LJ, Christian BJ, Wu S-Q, Meisner LF (1988) Neoplastic transformation of SV40-immortalized human urinary tract epithelial cells by in vitro exposure to 3-methylcholanthrene. Carcinogenesis (Lond) 9:1427Google Scholar
- 11.Reznikoff CA, Kao C, Messing EM, Newton M, Swaminathan S (1993) A molecular genetic model of human bladder carcinogenesis. Semin Cancer Biol 4:143Google Scholar