Journal of Comparative Physiology B

, Volume 158, Issue 3, pp 345–351 | Cite as

Characterization of a specific L-[3H]glutamic acid binding site on cilia isolated fromParamecium tetraurelia

  • Robin R. Preston
  • Peter N. R. Usherwood


Cilia isolated fromParamecium tetraurelia possess a specific, high affinity L-[3H]glutamic acid binding site, defined by an ED50 of 3.0×10−8M. The structural specificity of this site was probed by testing the competition between L-glutamate and various analogues for binding to cilia. The binding site is stereo-specific for L-glutamic acid, and requires the presence of all three ionizable groups on the glutamate molecule for optimal ligand: receptor interaction.

Specific binding of L-[3H]glutamic acid to cilia is rapid in onset but transient, reaching peak values within 6 min, and then declining thereafter. This transience may represent a form of sensory adaptation during prolonged exposure to the ligand.


Glutamate Binding Site Human Physiology Glutamic Acid Specific Binding 
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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Robin R. Preston
    • 1
  • Peter N. R. Usherwood
    • 1
  1. 1.Department of ZoologyUniversity of NottinghamNottinghamUK

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