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Virchows Archiv A

, Volume 402, Issue 3, pp 275–287 | Cite as

Fibroblast changes in cutaneous ageing

  • M. T. Pieraggi
  • M. Julian
  • H. Bouissou
Article

Summary.

With ageing there are progressive modifications in the connective tissue of the dermis. In ten young subjects the collagenous bundles are thick and the fibroblasts are active cells in close contact with collagen fascicles. In ten elderly subjects collagen fibres are fragmented and the fibroblasts are quiescent, without any contact with collagen.

In ageing the most important lesion is the destruction of the relationship between fibroblasts and interstitial matrix. A role for fibronectin in this adhesion is suggested: in old subjects the papillary network of fibronectin is poorly developed. Furthermore, in the fibroblast we can see architectural changes in the cytoskeleton; this modification breaks up the cytoskeleton - plasma membrane - fibronectin unit and explains the secretory and metabolic changes observed in ageing, the dysfunction of the cell-interstitial matrix unit, and also the structural changes.

Key words

Fibroblast Ageing Dermis 

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References

  1. Amblard P, Zambelli P (1980) Tissu conjonctif: les collagènes. In: Meynadier J (ed) de la Porte Verte: Précis de physiologic cutanée. Paris, p 137Google Scholar
  2. Bershadsky AD, Gelfand VI, Suitkina TM, Tint IS (1979) Cold stable microtubule in the cytoplasm of mouse embryo fibroblasts. Cell Biol Int Rep 3:45–50Google Scholar
  3. Bershadsky AD, Gelfand VI, Svitkina TM, Tint IS (1980) Destruction of microfilaments bundles in mouse embryo fibroblasts treated with inhibitors of energy metabolism. Exp Cell Res 127:421–429Google Scholar
  4. Bouissou H, Fabre MTh, Julian M, Rumeau JL, Huron R (1970) La biopsie cutanée permet-elle de connaître l'état de la paroi vasculaire? Rev Eur Et Clin Biol 15:444–453Google Scholar
  5. Bouissou H, Pieraggi MTh, Julian M (1971) Le fibroblaste cutané au cours du vieillissement et de certains états pathologiques. Ann Anat Pathol 16:373–386Google Scholar
  6. Bouissou H, Pieraggi MTh, Julian M, Douste-Blazy L (1973) Cutaneous ageing; its relation with arteriosclerosis and atheroma. Front Matrix Biol 1:190–211Google Scholar
  7. Bouissou H, De Graeve J, Solera ML, Thiers JC, Wulfert E, Enjalbert A, Puel P (1981) Apoprotéine B et cholestérol cutanés et sériques. Paroi Artérielle/Arterial Wall 7:22–28Google Scholar
  8. Brown MS, Kovanen PT, Goldstein JL (1981) Regulation of plasma cholesterol by lipoprotein receptors. Science 212:628–633Google Scholar
  9. Dessau W, Sasse J, Timpl R, Jilek F, von der Mark F (1978) Synthesis and extracellular deposition of fibronectin in chondrocyte cultures, response to the removal of extra-cellular cartilage matrix. J Cell Biol 79:342–347Google Scholar
  10. Engvall E, Ruoslahti E (1977) Binding of soluble form of fibroblast surface protein, fibronectin, to collagen. Int J Cancer 20:1–5Google Scholar
  11. Fleischmajer R, Dessau W, Timpl R, Krieg T, Leiderschmidt C, Wiestner M (1980a) Immuno-fluorescence analyses of collagen, fibronectin and basement membrane protein in scleroderma skin. J Invest Dermatol 75:270–274Google Scholar
  12. Fleischmajer R, Gay S, Pettish J, Cesarini JP (1980b) Immunoelectron microscopy of type III collagen in normal and scleroderma skin. J Invest Dermatol 75:189–191Google Scholar
  13. Fyrand O (1979) Studies on fibronectin in the skin. Br J Dermatol 101:263–270Google Scholar
  14. Gay ST, Muller PK, Meigel WN, Kuhnk (1976) Polymorphie des kollagens Neue Aspekte für Struktur und Funktion des Bindegewebes. Hautarzt 27:196–205Google Scholar
  15. Golstein S (1979) Studies on age related diseases in cultured skin fibroblasts. J Invest Dermatol 73:19–23Google Scholar
  16. Hayflick L (1974) Le vieillissement des cellules humaines. Triangle 14:129–139Google Scholar
  17. Junqueira LCU, Cossermelli W, Brentani RR (1978) Differential staining of collagens type I, II and III by sirius red and polarization microscopy. Arch Histol Jpn 41:267–274Google Scholar
  18. Junqueira LCU, Bignolas G, Brentani RR (1979) Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections. Histochemical J 11:447–455Google Scholar
  19. Kelley RO, Skipper BE (1977) Development of the aging cell surface: variation in the distribution of intramembrane particles with progressive age of human diploid fibroblast. J Ultrastr Res 59:113–118Google Scholar
  20. Keski OJ, Sen A, Todaro GS (1980) Direct association of fibronectin and actin molecules in vitro. J Cell Biol 85:527–533Google Scholar
  21. Mc Donagh J (1981) Fibronectin a molecular glue. Arch Pathol Lab Med 105:393–396Google Scholar
  22. Maurel E, Bouissou H, Pieraggi MTh, Julian M (1980) Age dependant biochemical changes in dermal connective tissue. Relationship to histological and ultrastructural observations. Connect Tissue Res 8:33–39Google Scholar
  23. Meigel WN, Gay S, Weber L (1977) Dermal architecture and collagen type distribution. Arch Dermatol Res 259:1–10Google Scholar
  24. Mosher DF, Furcht LT (1981) Fibronectin: review of its structure and possible functions. J Invest Dermatol 77:175–180Google Scholar
  25. Pena SDJ, Hughes RC (1978) Fibroblast to substratum contacts mediated by the different forms of fibronectin. Cell Biol Intern Rep 2:339–344Google Scholar
  26. Pena SDJ (1980) A new technique for the visualization of the cytoskeleton in cultured fibro- blasts with coomassie blue R 250. Cell Biol Intern Rep 4:149–153Google Scholar
  27. Pieraggi MTh, Julian M, Pasqual M, Bouissou H (1982) Localisation de l'apoprotéine B dans le derme humain. Nouvelle Presse Med 11:3551–3554Google Scholar
  28. Robert L, Robert B, Moczar E, Moczar M (1972) Les glycoprotéines de structure du tissu conjonctif. Pathol Biol 20:1001–1005Google Scholar
  29. Robert B, Robert L (1974) Le vieillissement du tissu conjonctif. Triangle 14:163–171Google Scholar
  30. Robert L (1980) Tissu conjonctif introduction. In: Meynadier J (ed) de la Porte Verte: Precis de physiologie cutanée. Paris, p 131Google Scholar
  31. Ruoslahti E, Engvall E, Hayman EG (1981) Fibronectin: current concepts of its structure and functions. Coll Res 1:95–128Google Scholar
  32. Ruoslahti E, Pierschbacher M, Engvall E, Oldberg A, Hayman EG (1982) Molecular and biological interactions of fibronectin. J Invest Dermatol 79:655–685Google Scholar
  33. Vogel KG, Kelley R, Stewart C (1981) Loss of organized fibronectin matrix from the surface of aging diploid fibroblast. Mech Ageing and Develop 16:295–302Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • M. T. Pieraggi
    • 1
  • M. Julian
    • 1
  • H. Bouissou
    • 1
  1. 1.Service d'Anatomie PathologiqueC.H.U. RangueilToulouse CedexFrance

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