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Journal of Comparative Physiology B

, Volume 158, Issue 6, pp 763–770 | Cite as

Temperature effects on intra- and extracellular acid-base status in the American locust,Schistocerca nitens

  • Jon M. Harrison
Article

Summary

This study examined the effect of temperature on hemolymph and intracellular acid-base status in the locust,Schistocerca nitens. Hemolymph pH decreased with temperature by 0.017 pH units·°C−1 above 25°C, but was stable at lower temperatures. Average intracellular pH (pHi, calculated from CO2 distribution) decreased by approximately 0.018 pH units·°C−1, in accordance with predictions for preservation of relative alkalinity and protein charge state. DMO was found to be unsuitable for use as an in vivo pHi marker in locusts due to rapid metabolism and excretion of this compound. Hemolymph pH regulation when temperature changed was accomplished by a combination of variation ofPCO2 and [HCO 3 ], with changes in [HCO 3 ] predominating. Digestive tracts contained a large portion of total body water (over 30%), and total body CO2 (over 40%). Variation in [HCO 3 ] may dominate pH regulation in locusts because of the relatively large size of the digestive tract and its powerful acid-base transporting capacities.

Keywords

Body Water Human Physiology Charge State Temperature Effect Digestive Tract 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

Ccar

carcass total CO2

Chem

hemolymph total CO2

Cin

intracellular total CO2

Ctot

total CO2

DMO

5,5 dimethyl-2,4-oxazolidinedione

ECW

extracellular water mass

ECWF

fraction of total body water which is extracellular

ICW

intracellular water mass

ICWF

fraction of total body water which is intracellular

PCA

perchloric acid

S

dissolved CO2

TBW

total body water

TV

tracheal volume

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Jon M. Harrison
    • 1
  1. 1.Department of Environmental, Population, and Organismic BiologyUniversity of ColoradoBoulderUSA

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