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Histochemistry

, Volume 67, Issue 3, pp 233–242 | Cite as

Potassium binding sites in muscle: Electron microscopic visualization of K, Rb, and Cs in freeze-dired preparations and autoradiography at liquid nitrogen temperature using86Rb and134Cs

  • L. Edelmann
Article

Summary

Normal frog sartorius muscles and muscles in which a major portion of the intracellular K+ was reversibly replaced by Rb+ or Cs+ were frozen, freeze-dried and embedded without chemical fixation or staining. Dry-cut sections of these preparations reveal striation patterns with higher contrast than those of wet-cut sections of the same preparation. The results suggest that in the living state the alkali metal ions are mainly localized in the A bands and Z lines of myofibrils. This idea is confirmed by a new autoradiographic technique by means of which the distribution of Rb+ and Cs+ in frozen-hydrated single muscle fibers has been investigated. The findings support the association-induction hypothesis according to which most cell K+ and other alkali-metal ions are not free in cell water but are adsorbed to beta- and gamma-carboxyl groups of cell proteins.

Keywords

Alkali Metal Cell Protein High Contrast Major Portion Liquid Nitrogen Temperature 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • L. Edelmann
    • 1
  1. 1.Medizinische Biologie, Fachbereich Theoretische MedizinUniversität des SaarlandesHomburg/SaarGermany

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