Acta Neuropathologica

, Volume 9, Issue 2, pp 146–157 | Cite as

Histological studies on the effect of actinomycin D on retrograde nerve cell reaction in the facial nucleus of mice

  • Ansgar Torvik
  • Anna Heding
Original Investigations


The retrograde reaction in the facial nucleus of mice has been studied after intracerebral injection of actinomycin D. Normally these neurons show increasing cytoplasmic basophilia and decreasing size of the Nissl granules during the first days after section of the facial nerve. The nerve cell diameter increases between the 2nd and 4th day after section of the nerve.

After intracerebral injection of actinomycin D the nucleolus of all neurons disintegrates rapidly while the cytoplasm shows only slight changes. The Nissl substance of normal neurons appears well preserved after 2 days' treatment.

When actinomycin is given at the time of section of the facial nerve, all aspects of retrograde reaction is blocked during the next 48 hours. The findings suggest that the dispersion of the Nissl substance during retrograde reaction is mediated through induced enzyme synthesis.

When the first injection of actinomycin is given 2 days after section of the nerve the neurons on the operated side show extreme chromatolysis 2 days later. This may indicate a more rapid turnover rate of the RNA of the finely dispersed cytoplasmic basophilic particles than of the large Nissl granules of normal neurons.


Facial Nerve Actinomycin Intracerebral Injection Facial Nucleus Normal Neuron 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Die retrograden Zellveränderungen im Facialiskern der Maus wurden nach intracerebraler Injektion von Actinomycin D untersucht. Normalerweise zeigen diese Neurone erhöhte Basophilie des Cytoplasmas und abnehmende Größe der Nissl-Schollen während der ersten Tage nach Durchtrennung des Nerven. Der Nervenzelldurchmesser nimmt während des 2.–4. Tages nach der Nervendurchtrennung zu.

Nach intracerebraler Injektion von Actinomycin D kommt es zur raschen Desintegration der Nucleoli sämtlicher Neurone, während das Cytoplasma nur geringe Veränderungen aufweist. Die Nissl-Substanz der normalen Neurone erscheint 2 Tage nach der Behandlung gut erhalten.

Wird Actinomycin zum Zeitpunkt der Durchtrennung des N. facialis verabreicht, so sind alle Veränderungen der retrograden Zellveränderung während der folgenden 48 Std blockiert. Diese Befunde weisen darauf hin, daß die Auflösung der Nissl-Substanz im Rahmen der retrograden Zellreaktion durch eine induzierte Enzymsynthese vermittelt wird.

Wird die erste Actinomycin-Injektion 2 Tage nach der Nervendurchtrennung verabreicht, so zeigen die Neurone der durchtrennten Seite nach 2 Tagen extreme Chromatolyse. Das könnte auf eine raschere Übertragungsrate von RNS auf die fein verteilten basophilen Partikeln des Cytoplasmas als auf die großen Nissl-Granula der normalen Neurone hinweisen.


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Copyright information

© Springer-Verlag 1967

Authors and Affiliations

  • Ansgar Torvik
    • 1
  • Anna Heding
    • 1
  1. 1.Department of PathologyUllevål HospitalOslo

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