Acta Neuropathologica

, Volume 6, Issue 1, pp 61–69

Effect of Echis colorata venom inoculation on the nervous system of the dog and guinea pig

  • U. Sandbank
  • M. Djaldetti
Original Investigations

Summary

Dogs and guinea pigs injected with Echis colorata venom showed the following lesions in the nervous system: 1. Massive stain of nervous tissue by Trypan blue and Fluorescite indicating break down of the blood brain barrier (guinea pigs). 2. Diffusion of adenosine triphosphatase activity in the vicinity of blood vessels. 3. Increase in number of lysosomes in neurons with spread into dendrites and axons. 4. Appearance of cytolysomes within the cytoplasm of neurons. 5. Marked thiamine pyrophosphatase activity of oligodendroglia.

It is suggested that the break down of the blood brain barrier is due to damage of the capillary basement membrane. The massive thiamine pyrophosphatase activity of the oligodendroglia is supposed to be secondary to the blood brain barrier defect. The distribution of lysosomes and Golgi apparatus in the neurons seems to be related to the disturbance of the blood brain barrier.

Zusammenfassung

Hunde und Meerschweinchen, die mit Gift der Schlange Echis colorata injiziert wurden, zeigten folgende Läsionen im Nervensystem: 1. Massive Anfärbung des Nervengewebes durch Trypanblau und Fluorescite, was auf den Zusammenbruch der Blut-Hirn-Schranke hinweist (Meerschweinchen). 2. Diffusion der Adenosin-Triphosphatase-Aktivität in die Umgebung der Blutgefäße. 3. Numerische Zunahme der Lysosomen in den Neuronen mit Ausbreitung in Dendriten und Axone. 4. Auftreten von Cytolysomen im Cytoplasma der Neurone. 5. Deutliche Thiamin-Pyrophosphatase-Aktivität der Oligodendroglia.

Es wird angenommen, daß der Zusammenbruch der Blut-Hirn-Schranke einer Schädigung der Basalmembran der Capillaren entspricht. Die starke Thiamin-Pyrophosphatase-Aktivität der Oligodendroglia ist wahrscheinlich sekundär zum Blut-Hirn-Schrankendefekt. Die Verteilung der Lysosomen und Golgi-Apparate in den Neuronen scheint in Beziehung zur Störung der Blut-Hirn-Schranke zu stehen.

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References

  1. 1.
    Barron, K. D.: Histochemical study of necrosis produced by intra-cerebral injection of diphteria toxin. Neurology (Minneap.)11, 714–723 (1961).Google Scholar
  2. 2.
    Becker, N. H., andK. D. Barron: The cytochemistry of anoxic and anoxi-ischemic encephalopathy in rats. Alteration in neuronal lysosomes identified by acid phosphatase activity. Amer. J. Path.38, 161–175 (1961).Google Scholar
  3. 3.
    —, andA. B. Novikoff: The localisation of enzyme activities in the rat brain. J. biophys. biochem. Cytol.8, 649–663 (1960).Google Scholar
  4. 4.
    —, andS. Goldfischer: A cytochemical study of the neuronal Golgi apparatus. Arch. Neurol. (Chic.)5, 497–503 (1961).Google Scholar
  5. 5.
    —, andU. Sandbank: The neuronal Golgi Apparatus and lysosomes in neonatal rats. J. Histochem. Cytochem.12, 483–485 (1964).Google Scholar
  6. 6.
    Dempsey, E. W., andG. B. Wislocki: An electron microscopic study of the blood brain barrier in the rat employing silver nitrate as a vital stain. J. biophys. biochem. Cytol.1, 245–256 (1955).Google Scholar
  7. 7.
    Gitter, S.: Toxicology and biochemistry of the venoms of some Israeli snakes. Dapim Refuiim21, 610–613 (1962).Google Scholar
  8. 8.
    —, andJ. Casper: Studies on the venom of Echis colorata. Amer. J. trop. Med. Hyg.9, 391–399 (1960).Google Scholar
  9. 9.
    —, andN. Goldblum: Studies on the snake venoms of the Near East. Walterinnesia Aegyptia and Pseudocerastes fieldii. Amer. J. trop. Med. Hyg.11, 861–868 (1962).Google Scholar
  10. 10.
    Gomori, G.: Microscopic Histochemistry: Principles and practice. Chicago: University of Chicago Press 1952.Google Scholar
  11. 11.
    Klatzo, I., J. Miquel, andR. Otenasek: The application of Fluorescein labelled serum proteins (FLSP) to the study of vascular permeability in the brain. Acta neuropath. (Berl.)2, 144–160 (1962).Google Scholar
  12. 12.
    Kochwa, S., Ch. Perlmutter, S. Gitter, J. Rechnic, andA. de Vries: Studies on Vipera palestinae venom, fractionation by ion exchange chromatography. Amer. J. trop. Med. Hyg.9, 374–380 (1960).Google Scholar
  13. 13.
    Lillie, R. D.: Histopathologic Technic and Practical Histochemistry. New York: The Blakinston Co. 1954.Google Scholar
  14. 14.
    Luse, S. A.: Ultrastructure of Brain and its Relation to Transport of Metabolites. InKorey, S. A.: Ultrastructure and Metabolism of the Nervous System. pp. 1–24 A.R.N.M.D., Vol. 40. Baltimore: Williams & Wilkins Co. 1962.Google Scholar
  15. 15.
    Moav, B., Ch. Moroz, andA. de Vries: Activation of the fibrinolytic system of the Guinea pig following inoculation of Echis colorata venom. Toxicon1, 109–112 (1963).Google Scholar
  16. 16.
    Novikoff, A. B., andE. Essner: Pathological changes in cytoplasmic organelles. Fed. Proc.21, 1130–1142 (1962).Google Scholar
  17. 17.
    Torack, R. M., andR. J. Barrnett: The fine structural localization of nucleoside phosphatase activity in the blood brain barrier. J. Neuropath. exp. Neurol.23, 46–59 (1964).Google Scholar
  18. 18.
    Wachstein, M., andE. Meisel: Histochemistry of hepatic phosphatase at a physiologic pH. Amer. J. clin. Path.27, 13–23 (1957).Google Scholar

Copyright information

© Springer-Verlag 1966

Authors and Affiliations

  • U. Sandbank
    • 1
    • 2
  • M. Djaldetti
    • 1
    • 2
  1. 1.Laboratory of Neuropathology, Department of Pathology of the Tel Aviv University, Beilinson HospitalPetah Tikva
  2. 2.Rogoff Medical Research Institute, Department of Experimental Biology of the Tel Aviv University, Beilinson HospitalPetah Tikva

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