Acta Neuropathologica

, Volume 41, Issue 1, pp 27–31

Cerebral tumors induced by transplacental ENU: Study of the different tumoral stages, particularly of early proliferations

  • D. Schiffer
  • M. T. Giordana
  • S. Pezzotta
  • C. Lechner
  • P. Paoletti
Original Investigations

Summary

Experimental cerebral tumors have been induced by transplacental ENU. A systematic diachronic study of the brains has been performed starting from the 15th day of extrauterine life. The tumoral lesions firstly appear as “early stage proliferations” or oligodendroglial foci and develop as glial micro-and macrotumors or as isomorphic and polymorphic oligodendrogliomas respectively.

Neurinomas appear later than glial tumors.

The duration of the different lesions is sketched out. The usefulness of the model for chemotherapy studies is discussed.

Key words

Experimental CNS tumors Transplacental ENU Early stage Tumor development 

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References

  1. Barker, M., Hoshino, T., Gurcay, O. Wilson, C. B., Nielsen, S. L., Downie, R., Eliason, J.: Development of an animal brain tumor model and its response to therapy with 1,3-bis(2-chlroethyl)-1-nitrosourea. Cancer Res.33, 976–983 (1973)Google Scholar
  2. Bigner, D. D., Pegram, C. N.: Virus-induced experimental brain tumors and putative associations of viruses with human brain tumors: a review. In: Advances in Neurology, Vol. 15: Neoplasia in the Central Nervous System. (Ed. R. A. Thompson and J. R. Green), p. 57–83. New York: Raven Press 1976Google Scholar
  3. Blakemore, W. F.: The ultrastructure of the subependymal plate in the rat. J. Anat. (London)104, 423–433 (1969)Google Scholar
  4. Bosch, D. A.: Short and long term effects of methyl-and ethylnitrosourea (MNU & ENU) on the developing nervous system of the rat. I. Acta Neurol. Scand.55, 85–105 (1977)Google Scholar
  5. Chen, T. T., Mealey, J., Jr.: Effects of cytosine arabinoside and 1,3-bis(2-chloroethyl)-1-nitrosourea on human glial tumor cells. Cancer Res.32, 558–564 (1972)Google Scholar
  6. Grossi-Paoletti, E., Paoletti, P., Schiffer, D., Fabiani, A.: Experimental brain tumors induced in rats by nitrosourea derivatives. Part II. Morphological aspects of nitrosoethylurea tumors obtained by transplacental induction. J. Neurol. Sci.11, 573–581 (1970)Google Scholar
  7. Jacobson, M.: Developmental neurobiology. In: Developmental biology series (Ed. J. D. Ebert), pp. 1–74 New York: Holt, Rinehart and Winston 1970Google Scholar
  8. Kleihues, P., Matsumoto, S., Wechsler, W., Zülch, K. J.: Morphologie und Wachstum der mit Äthylnitroso-Harnstoff transplazentar erzeugten Tumoren des Nervensystems. Verh. Dtsch. Ges. Path.52, 372–388 (1968)Google Scholar
  9. Koestner, A., Swenberg, J. A., Wechsler, W.: Transplacental production with ethylnitrosourea of neoplasms of the nervous system in Sprague-Dawley rats. Amer. J. Path.63, 37–56 (1971)Google Scholar
  10. Lantos, P. L.: The fine structure of the periventricular pleomorphic gliomas induced transplacentally by N-ethyl-N-nitrosourea in BD-IX rats. With a note on their origin. J. Neurol. Sci.17, 443–460 (1972)Google Scholar
  11. Mealey, J., Jr, Chen, T. T., Shupe, R.: Response of cultured human glioblastomas to radiation and BCNU chemotherapy. J. Neurosurg.41, 339–349 (1974)Google Scholar
  12. Rath, F. W., Schneider, H., van, Calker, H.: Histochemische Untersuchungen an Frühstadien experimenteller Hirntumoren. In: Experimentelle Neuroonkologie (Eds. D. Schreiber und W. Jänisch), S. 94–100. Leipzig: J. A. Barth 1974Google Scholar
  13. Schiffer, D., Giordana, M. T.: On the occurrence and significance of acid mucopolysaccharides in oligodendrogliomas experimentally induced in the rat by nitrosourea derivatives In: Experimentelle Neuroonkologie (Eds. D. Schreiber und W. Jänisch), S. 101–108. Leipzig: J. A. Barth 1974Google Scholar
  14. Schiffer, D., Giordana, M. T.: Acid mucopolysaccharides in experimental brain tumors. Proc. VII Intern. Congr. Neuropath. Budapest 1974. (Eds. S. Környey, S. Tariska and G. Gosztonyi), pp. 533–540. Amsterdam: Excerpta Medica 1975Google Scholar
  15. Schiffer, D., Giordana, M. T., Pezzotta, S., Paoletti, P.: Chemotherapeutic effects of some alkalylating derivatives of nitrosou rea on the development of tumors transplacentally induced in rats by ENU. Acta Neuropath. (Berlin)34, 21–31 (1976)Google Scholar
  16. Shapiro, W. R., Ausman, J. I., Rall, D. P.: Studies on the chemotherapy of experimental brain tumors: evaluation of 1,3-bis-(2-chloroethyl)-1-nitrosourea, cyclophosphamide, mithramycin and methotrexate. Cancer Res.30, 2401–2413 (1970)Google Scholar
  17. Stavrou, D.: Enzymhistochemische Befunde im subependymalen Glialager während der Entwicklung des Rattenhirns. Histochemie,34, 85–96 (1973)Google Scholar
  18. Swenberg, J. A.: Animal models for brain tumor research. Proc. Symp. V Intern. Congr. Neurol. Surg. Tokyo, 1973. pp. 116–122. Amsterdam: Excerpta Medica 1974Google Scholar
  19. Swenberg, J. A., Wechsler, W., Koestner, A.: The sequential development of transplacentally induced neuroectodermal tumors. J. Neuropath. exp. Neur.30, 202–203 (1971)Google Scholar
  20. Thompson, J. M., Jr., Maruyama, Y., Schwartz, S., Hahn, E.: Effect of chemotherapy and/or head irradiation in survival of mice with intracranial glioma. Radiol.101, 187–194 (1971)Google Scholar
  21. Wechsler, W., Kleihues, P., Matsumoto, S., Zülch, K. J., Ivankovic, S., Preusmann, R., Druckrey, H.: Pathology of experimental neurogenic tumors chemically induced during prenatal and postnatal life. Ann. N. Y. Acad. Sci.159, 360–408 (1969)Google Scholar
  22. Wheeler, G. P., Bowdon, B. J., Adamson, D. D. J., Vail, M. H.: Effects of 1,3-bis(2-chloroethyl)-1-nitrosourea and some chemically related compounds upon the progression of cultured H. Ep. No. 2 cells through the cell cycle. Cancer Res.30, 1817–1827 (1970)Google Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • D. Schiffer
    • 1
  • M. T. Giordana
    • 1
  • S. Pezzotta
    • 2
  • C. Lechner
    • 2
  • P. Paoletti
    • 2
  1. 1.II. Neurological ClinicUniversity of TurinTurinItaly
  2. 2.Neurosurgical ClinicUniversity of PaviaItaly

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