Acta Neuropathologica

, Volume 37, Issue 1, pp 13–19 | Cite as

Axon reaction in the red nucleus of the rat

Perikaryal volume changes and the time course of chromatolysis following cervical and thoracic lesions
  • D. A. Egan
  • B. A. Flumerfelt
  • D. G. Gwyn
Original Investigations

Summary

Two groups of 60 day old male Wistar Rats were subjected to right-sided rubro-spinal tractotomy at the fourth cervical and thirteenth thoracic vertebral levels respectively. Four animals in each group were sacrificed at each of the time intervals 1, 3, 4, 7, 14, 21 and 55 days. Counts of chromatolytic neurons from both groups of animals at time intervals ranging from 1–21 days established a time course for the chromatolytic response. Morphological observations showed a more severe central chromatolysis in the cervical group commencing at Day 3, and a much less severe central chromatolysis in the thoracic group starting at Day 4. By Day 21 the majority of the neurons of the cervical group were atrophic and by 55 days all were atrophic. The reaction following thoracic lesions was less intense and the maximum number of reacting neurons was observed at Day 7. Following this stage the neurons gradually returned to a normal morphological state which was complete by Day 55. Measurements of neuronal diameters from semi-thin Epon sections in the cervical group yielded statistically significant alterations in the perikaryal diameters of experimental neurons, demonstrating swelling at Days 1, 3 and 4 followed by shrinkage at Days 7, 14 and 21.

The findings of this study suggest that the severity of the chromatolytic reaction in intrinsic neurons is intimately related to the distance of the lesion from the neuronal soma, while the time of onset of chromatolysis varies with lesion distance but is not directly proportional to it.

Key words

Chromatolysis Axon reaction Intrinsic neurons Lesion distance Volume changes 

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References

  1. Barr, M. L., Hamilton, J. D.: A quantitative study of certain morphological changes in spinal motor neurons during axon reaction. J. comp. Neurol.89, 93–121 (1948)Google Scholar
  2. Beresford, W. A.: A discussion of retrograde changes in nerve fibres. Progr. Brain Res.14, 35–56 (1965)Google Scholar
  3. Bodian, D., Mellors, R. C.: The regenerate cycle of motor neurons with special reference to phosphatase activity. J. exp. Med.81, 469–488 (1945)Google Scholar
  4. Brattgård, S. O., Edström, J. E., Hydén, H.: The chemical changes in regenerating neurons. J. Neurochem.1, 316–325 (1957)Google Scholar
  5. Bucy, P. C.: Studies in degeneration of peripheral nerves. J. comp. Neurol.45, 129–159 (1928)Google Scholar
  6. Cajal, S. R., y: Degeneration and regeneration of the nervous system (Transl.) (ed. R. M. May), New York: Hafner 1928Google Scholar
  7. Cragg, B. G.: What is the signal for chromatolysis? Brain Res.23, 1–21 (1970)Google Scholar
  8. Edström, J. E.: Ribonucleic acid changes in the motor neurons of the frog during axon regeneration. J. Neurochem.5, 43–49 (1959)Google Scholar
  9. Fisher, R. A., Yates, F.: Statistical tables for biological, agricultural and medical research. 6th Edition. Edinburgh, Oliver and Boyd 1963Google Scholar
  10. Fry, F. J., Cowan, W. M.: A study of retrograde cell degeneration in the lateral mammillary nucleus of the cat, with special reference to the role of axonal branching in the preservation of the cell. J. comp. Neurol.144, 1–24 (1972)Google Scholar
  11. Geist, F. D.: Chromatolysis of efferent neurons. Arch. Neurol. Psychiat.29, 88–103 (1933)Google Scholar
  12. Gersh, I., Bodian, D.: Some chemical mechanisms in chromatolysis. J. Cell Physiol.21, 253–279 (1943)Google Scholar
  13. Glover, R. A.: Sequential cellular changes in the nodosal ganglion following section of the vagus nerve at two levels. Anat. Rec.157, 248 (Abstract) (1967)Google Scholar
  14. Humbertson, A.: A chronological study of the degenerative phenomena of dorsal root ganglia cells following section of the sciatic nerve. Anat. Rec.145, 244 (Abstract) (1963)Google Scholar
  15. Kirkpatrick, J. B.: Chromatolysis in the hypoglossal nucleus of the rat: an electron microscopic analysis. J. comp. Neurol.132, 189–212 (1968)Google Scholar
  16. Koenig, H., Groat, R. A., Windle, W. F.: A physiological approach to perfusion fixation of tissues with formalin. Stain Technol.20, 13–22 (1945)Google Scholar
  17. Lewis, P. R., Shute, C. C. D.: The distribution of cholinesterase in cholinergic neurons demonstrated with the electron microscope. J. Cell Sci.1, 381–390 (1966)Google Scholar
  18. Lieberman, A. R.: The axon reaction: A review of the features of perikaryal responses to axon injury. Int. Rev. Neurobiol.14, 49–124 (1971)Google Scholar
  19. Liu, C. N.: Time pattern in retrograde degeneration after trauma of central nervous system of mammals. In: Regeneration in the Central Nervous System (ed. W. Windle), pp. 84–93. Springfield, Ill.: Ch. C. Thomas 1955Google Scholar
  20. Marinesco, G.: La cellule nerveuse. Paris: Doin 1909Google Scholar
  21. Matthews, M. R., Raisman, G.: A light and electron microscopic study of the cellular response to axonal injury in the superior cervical ganglion of the rat. Proc. roy. Soc. B181, 43–79 (1972)Google Scholar
  22. Means, E. D., Barron, K. D.: Histochemical and histological studies of axon reaction in feline motor neurons. J. Neuropath. exp. Neurol.31, 221–246 (1972)Google Scholar
  23. Murray, M., Grafstein, B.: Changes in morphology and amino acid incorporation of regenerating goldfish optic neurons. Exp. Neurol.23, 544–560 (1969)Google Scholar
  24. Nissl, F.: Über die Veränderungen der Ganglienzellen am Facialiskern des Kaninchens nach Ausreißung der Nerven. Allg. Z. Psychiat.48, 197–198 (1892)Google Scholar
  25. Nittono, K.: On bilateral effects from the unilateral section of branches of the nervus trigeminus in the albino rat. J. comp. Neurol.35, 133–161 (1923)Google Scholar
  26. Pannese, E.: Investigations on the ultra-structural changes of the spinal ganglion neurons in the course of axon regeneration and cell hypertrophy. I. Changes during axon regeneration. Z. Zellforsch.60, 711–740 (1963a)Google Scholar
  27. Pannese, E.: Investigations on the ultrastructural changes of the spinal ganglion neurons in the course of axon regeneration and cell hypertrophy. II. Changes during cell hypertrophy and comparison between the ultrastructure of nerve cells of the same type under different conditions. Z. Zellforsch.61, 561–586 (1963b)Google Scholar
  28. Peters, A.: The fixation of central nervous tissue and the analysis of electronmicrographs of the neuropil with special reference to the cerebral cortex. In: Contemporary Research Methods in Neuroanatomy (eds. W. J. H. Nauta, S. O. E. Ebbesson), pp. 56–76. New York: Springer 1970Google Scholar
  29. Richardson, K. C., Jarett, L., Finke, E. H.: Embedding in epoxy resins for ultra-thin sectioning in electron microscopy. Stain Technol.35, 313–323 (1960)Google Scholar
  30. Smith, R. S.: Centripetal movement of particles in myelinated axons. Cytobios3, 259–262 (1971)Google Scholar
  31. Torvik, A., Heding, A.: Histological studies on the effects of actinomycin D on retrograde nerve cell reaction in the facial nucleus of mice. Acta neuropath. (Berl.)9, 146–157 (1967)Google Scholar
  32. Torvik, A., Heding, A.: Effect of actinomycin D on retrograde nerve cell reaction. Further observations. Acta neuropath. (Berl.)14, 62–71 (1969)Google Scholar
  33. Vaughn, J. E., Peters, A.: Aldehyde fixation of nerve fibres. J. Anat. (Lond.)100, 687 (1966)Google Scholar
  34. Watson, W. E.: An autoradiographic study of the incorporation of nucleic-acid precursors by neurones and glia during nerve regeneration. J. Physiol. (Lond.)180, 741–753 (1965)Google Scholar
  35. Watson, W. E.: Observations on the nucleolar and total cell body nucleic acid of injured nerve cells. J. Physiol. (Lond.)196, 655–676 (1968)Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • D. A. Egan
    • 1
  • B. A. Flumerfelt
    • 1
  • D. G. Gwyn
    • 1
  1. 1.Department of Anatomy, Health Sciences CentreThe University of Western OntarioLondonCanada
  2. 2.School of PhysiotherapyDalhousie UniversityHalifaxCanada
  3. 3.Department of AnatomyDalhousie UniversityHalifaxCanada

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