Journal of Comparative Physiology B

, Volume 154, Issue 2, pp 159–166 | Cite as

Effect of cold acclimation on Na+/K+ transport in hamster liver cells

  • B. A. Horwitz
  • S. D. Clark
  • J. Hamilton
  • J. Guttas
Article
Accepted:

Summary

The effects of prolonged cold exposure of Syrian hamsters on liver membrane (Na+/K+-ATPase activity and on liver intracellular K+ levels was examined. Membrane preparations from cold-acclimated hamsters (6°C for 3 weeks) exhibited significantly higherVmax values for (Na+/K+)-ATPase and significantly greater ouabain binding. These data support the view that in the liver of these cold-exposed hamsters, there is an increase in the number of operational pumps. The fact that the intact liver cells (isolated via liver perfusion) from the cold-acclimated hamsters: (a) did not have higher concentrations of intracellular K+ (despite the presence of more operational pumps); and (b) exhibited greater rates of K+ loss when the pumps were inhibited by maximal ouabain suggests that the K+ leak across the liver cell plasma membrane is increased in the cold-acclimated hamsters. Although the physiological significance of these results needs further evaluation, these membrane changes may be of adaptive value for hibernation.

Keywords

Liver Cell ATPase Activity Ouabain Cold Acclimation Cold Exposure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

CA

cold-acclimated

Pi

inorganic phosphate

KRB

Krebs-Ringer-bicarbonate buffer

BSA

bovine serum albumin

ECF

extracellular fluid

ICF

intracellular fluid

dcs

dry cell solid

N

nitrogen

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • B. A. Horwitz
    • 1
  • S. D. Clark
    • 1
  • J. Hamilton
    • 1
  • J. Guttas
    • 1
  1. 1.Department of Animal PhysiologyUniversity of CaliforniaDavisUSA

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