Marine Biology

, Volume 120, Issue 3, pp 415–420 | Cite as

Inheritance of a nuclear DNA polymorphism assayed in single bivalve larvae

  • H. B. S. M. Côrte-Real
  • P. W. H. Holland
  • D. R. Dixon
Article

Abstract

We examined the inheritance of theMytilus edulis CaM-1 Intron 3 locus, a non-coding DNA locus with two potentially neutral length-variants. The polymerase chain reaction (PCR) was used to determine theCaM-1 genotype for 799 larvae obtained from 11 laboratory crosses. Larvae were typed singly only 8 to 24 h after fertilization. We find evidence that each allele can be inherited from either sex, that there are no barriers to fertilization between gametes of different genotypes, and that most larvae have genotypes compatible with Mendelian inheritance of the locus. Deviations from expected genotype frequencies were found in some crosses; we suggest that a contributing factor is aneuploidy in early larvae, but a major cause is more likely to be selection at a locus linked toCaM-1, occurring under the artificial laboratory culture conditions. This study demonstrates the feasibility of applying molecular genetic techniques to early larval stages of marine bivalves, and presents a new non-destructive biopsy method for DNA analysis from living adult mussels.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Beaumont AR (1991) Genetic studies of laboratory reared mussels,Mytilus edulis: heterozygote deficiencies, heterozygosity and growth. Biol J Linn Soc 44:273–285Google Scholar
  2. Borsa P, Zainuri M, Delay B (1991) Heterozygote deficiency and population structure in the bivalveRuditapes decussatus. Heredity, Lond 66: 1–8Google Scholar
  3. Chakraborty R (1989) Can molecular imprinting explain heterozygote deficiency and hybrid vigor? Genetics, Austin, Tex 122: 713–717Google Scholar
  4. Clark AG, Lanigan CMS (1993) Prospects for estimating nucleotide divergence with RAPDs. Molec Biol Evolut 10:1096–1111Google Scholar
  5. Côrte-Real HBSM, Dixon DR, Holland PWH (1994) Intron-targeted PCR: a new approach to survey neurtral DNA polymorphism in bivalve populations. Mar Biol 120:407–413Google Scholar
  6. Dixon DR (1982) Aneuploidy in mussel embryos (Mytilus edulis L.) originating from a polluted dock., Mar Biol Lett 3: 155–161Google Scholar
  7. Dixon DR, Flavell N (1986) A comparative study of the chromosomes ofMytilus edulis, andMytilus galloprovincialis. J mar biol Ass UK 66:219–228Google Scholar
  8. Ellesworth DL, Rittenhouse KD, Honeycutt RL (1993) Artefactual variation in randomly amplified polymorphic DNA banding patterns. BioTechniques 14:214–217Google Scholar
  9. Faibrother JE, Beaumont AR (1993) Heterozygote deficiencies in a cohort of newly settledMytilus edulis spat. J mar biol Ass UK 73:647–653Google Scholar
  10. Foltz DW (1986) Segregation and linkage studies at allozyme loci in pair crosses of the oysterCrassostrea virginica. Biochem Genet 24:941–956Google Scholar
  11. Gosling EM (1992) Genetics ofMytilus. In: Gosling EM (ed) The musselMytilus: ecology, physiology, genetics and culture. Elsevier, Amsterdam, pp 309–382Google Scholar
  12. Hadrys H, Balick M, Schierwater B (1992) Applications of random amplified polymorphis DNA (RAPD) in molecular ecology. Molec Ecol 1:55–63Google Scholar
  13. Higuchi R (1989) Simple and rapid preparation of samples for PCR. In: Erlich HA (ed) PCR Technology. Stockton Press, New York, pp 31–38Google Scholar
  14. Hu Y-P, Lutz RA, Vrijenhoek RC (1992) Electrophoretic identification and genetic analysis of bivalve larvae. Mar Biol 113:227–230Google Scholar
  15. Hvilsom MM, Theisen BF (1984) Inheritance of allozyme variations through crossing experiments with the blue mussel,Mytilus. edulis L. Hereditas 101:1–7Google Scholar
  16. Koehn PK (1991) The genetics and taxonomy of species in the genusMytilus. Aquaculture Amsterdam 94: 125–145Google Scholar
  17. Lewin B (1990) Genes. IV. Oxford University Press, OxfordGoogle Scholar
  18. Mallet AL, Haley LE (1983) Effects of inbreeding on larval and spat performance in the American oyster. Aquaculture, Amsterdam 33:229–235Google Scholar
  19. Mallet AL, Zouros E, Gartner-Kepkay KE, Freeman KR, Dickie LM (1985) Larval viability and heterozygote deficiency in populations of marine bivalves: evidence from pair matings of mussels. Mar Biol 87:165–172Google Scholar
  20. Olson RR, Runstadler JA, Kocher TD (1991) Whose larvae? Nature, Lond 351:357–358Google Scholar
  21. Orrego C (1990) Organizing a laboratory for PCR work. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols. Academic Press, San Diego, pp 447–454Google Scholar
  22. Schlotterer C, Tautz D (1992) Slippage, synthesis of simple sequence DNA. Nucleic Acids Res 20:211–215Google Scholar
  23. Zouros E, (1987) On the relation between heterozygosity and heterosis: an evaluation of the evidence from marine molluscs. In: Rattazzi MC, Scandalios JG, Whitt GS (eds) Isozymes: current topics in biological and medical research. Vol 15. Alan R Liss, Inc, New York, pp 225–270Google Scholar
  24. Zouros E, Foltz DW (1987) The use of allelic isozyme variation for the study of heterosis. In: Rattazzi MC, Scandalios JG, Whitt GS, (eds) Isozymes: current topics in biological and medical research. Vol 13. Alan R Liss, Inc, New York, pp 1–59Google Scholar
  25. Zouros E, Romero-Dorey M, Mallet AL (1988) Heterozygosity and growth in marine bivalves: further data and possible explanations. Evolution 42: 1332–1341Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • H. B. S. M. Côrte-Real
    • 1
    • 2
  • P. W. H. Holland
    • 1
  • D. R. Dixon
    • 2
  1. 1.Department of ZoologyUniversity of OxfordOxfordEngland
  2. 2.Plymouth Marine LaboratoryPlymouthEngland
  3. 3.Department of Pure and Applied Zoology. School of Animal and Microbial SciencesUniversity of ReadingReadingEngland

Personalised recommendations