Zusammenfassung
Ein einfaches Reinigungsverfahren für das Peptidhormon Relaxin wird beschrieben. Mit ihm ist eine größere Menge an hochgereinigtem Relaxinpräparat von 700 GPU/mg Protein zu erlangen. Erstmalig wurden dazu der Ionenaustauscher Amberlite IRA-410 und anschließend Sephadex G-25 fine verwendet.
Schlüsselwörter
Relaxin Reinigung Ionenaustauscher Physikalische und chemische EigenschaftenPurification of the peptide hormone relaxin
Summary
A simple procedure for the purification of the peptide hormone relaxin is discrebed. It allows prepare with good yield highly purified relaxin of an activity of 700 GPU/mg protein.
For the first time ion-exchange chromatography by Amberlite IRA-410 and subsequent gel filtration on sephadex G-25 fine were used as purifications steps.
Key words
Relaxin Purification Ion-exchange chromatography Physical and chemical propertiesPreview
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Copyright information
© J. F. Bergmann-Verlag 1977