Pflügers Archiv

, Volume 390, Issue 3, pp 265–269 | Cite as

Induction of extrajunctional acetylcholine sensitivity of rat EDL muscle by peptidic component of peripheral nerve

  • F. Vyskočil
  • I. Syrový
  • Z. Prusík
Excitable Tissues and Central Nervous Physiology
Received:
Accepted:

Abstract

The induction of extrajunctional ACh-sensitivity was studied electrophysiologically 4 days after implantation of segments of the sciatic nerve, segments of dorsal and ventral spinal roots and silastic plates, containing some components of peripheral nerve onto surface fibres of innervated rat extensor digitorum longus muscle. It has been found that extrajunctional ACh-sensitivity can be induced by a piece of rat sciatic nerve and by peptidic material of about 8,000 and 10,000 mol. wt.

No sensitivity was induced by segments of ventral and dorsal roots, by a piece of rabbit sciatic nerve and by rat sciatic nerve sectioned 7 days prior to implantation.

The active nerve fractions lost its inducing potency after pepsin pre-treatment and irradiation with UV-light. Peripheral nerve probably contains some specific factor, peptidic in nature, which is able to induce the ACh-sensitivity of muscle fibre membrane.

Key words

Extrajunctional ACh sensitivity Peripheral nerve peptides Spinal cord roots Nerve fractionation 
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References

  1. Allt G (1976) Pathology of the peripheral nerve. In: Landon DN (ed) The peripheral nerve. Chapman and Hall, London, pp 666–739Google Scholar
  2. Arnow LE (1936) Effects produced by the irradiation of proteins and amino acids. Physiol Rev 16:671–685Google Scholar
  3. Blunt RJ, Josen R, Vrbová G (1975) Inhibition of cell division and the development of denervation hypersensitivity in skeletal muscle. Pflügers Arch 355:189–204Google Scholar
  4. Cangiano A, Lutzemberger L (1977) Partial denervation affects both denervated and innervated fibres in the mammalian skeletal muscle. Science 196:542–545Google Scholar
  5. Del Castillo J, Katz B (1955) On the localization of acetylcholine receptors. J Physiol 128:157–181Google Scholar
  6. Fatt P, Katz B (1951) An analysis of the endplate potential recorded with an intracellular electrode. J Physiol 115:320–370Google Scholar
  7. Gamble HJ (1964) Comparative electron microscope observation on the connective tissue of a peripheral nerve and a spinal root in the rat. J Anat 98:17–25Google Scholar
  8. Gamble HJ (1976) Spinal and cranial nerve roots. In: Landon DN (ed) The peripheral nerve. Chapman and Hall, London, pp 330–354Google Scholar
  9. Guth L (1968) “Trophic” influences of nerve on muscle. Physiol Rev 48:645–687Google Scholar
  10. Guth L, Richman E, Barrett Ch, Warnick JE, Albuquerque EX (1980) The mechanism by which degenerating peripheral nerve produces extrajunctional acetylcholine sensitivity in mammalian skeletal muscle. Exp Neurology 68:465–476Google Scholar
  11. Gutmann E (1976) Neurotrophic relations. Ann Rev Physiol 38:177–216Google Scholar
  12. Hannig K (1961) Die trägerfreie kontinuierliche Elektrophorese und ihre Anwendung. Z Anal Chem 181:244–250Google Scholar
  13. Hasegawa S, Kuromi H (1978) Ventral part of spinal cord contains the neurotrophic factor for the action potential of cultured muscle. Brain Res 157:153–156Google Scholar
  14. Jessell TM, Siegel RE, Fischbach GD (1979) Induction of acetylcholine receptors on cultured skeletal muscle by a factor extracted from brain and spinal cord. Proc Natl Acad Sci USA 76:5397–5401Google Scholar
  15. Jirmanová I, Thesleff S (1972) Ultrastructural study of experimental muscle degeneration and regeneration in the adult rat. J Zellforsch 131:77–97Google Scholar
  16. Jones R (1979) Factors affecting the distribution of acetylcholine receptors in innervated and denervated skeletal muscle fibres. In: Tuček S (ed) The cholinergic synapse. Progress in Brain Res 49:391–402Google Scholar
  17. Jones R, Vrbová G (1974) Two factors responsible for the development of denervation hypersensitivity. J Physiol 236:517–538Google Scholar
  18. Jones R, Vyskočil F (1975) An electrophysiological examination of the changes in skeletal muscle fibres in response to degenerating nerve tissue. Brain Res 88:309–317Google Scholar
  19. Lieben F (1927) Über die Zerstörung einiger Aminosäuren durch Belichtung”. Biochem Z 184:453–473Google Scholar
  20. Lømo T, Westgaard RH (1975) Further studies on the control of ACh sensitivity by muscle activity in the rat. J Physiol 252:603–626Google Scholar
  21. Markelonis G, Oh TH (1979) A sciatic nerve protein has a trophic effect on development and maintenance of skeletal muscle cells in culture. Proc Natl Acad Sci USA 76:2470–2474Google Scholar
  22. Miledi R (1960) The acetylcholine sensitivity of frog muscle fibres after complete or partial denervation. J Physiol 151:1–23Google Scholar
  23. Nastuk WL (1953) Membrane potential changes at single muscle endplate produced by transitory application of acetylcholine with an electrically controlled microjet. Fed Proc 12:102Google Scholar
  24. Oh TH (1975) Neurotrophic effects: Characterization of the nerve extract that stimulates muscle development in culture. Exp Neurol 46:432–438Google Scholar
  25. Oh TH (1976) Neurotrophic effects of sciatic nerve extracts on muscle development in culture. Exp Neurol 50:376–386Google Scholar
  26. Oh TH, Markelonis GJ (1978) Neurotrophic protein regulates muscle acetylcholineesterase in culture. Science 200:337–339Google Scholar
  27. Oh TH, Markelonis GJ, Reier PJ, Zalewski AA (1980) Persistence in degenerating sciatic nerve of substances having a trophic influence upon cultured muscle. Exp Neurol 67:646–654Google Scholar
  28. Podleski TR, Axelrod D, Ravdin P, Greengberg I, Johnson MM, Salpeter MM (1978) Nerve extract induces increase and redistribution of acetylcholine receptors on cloned muscle cells. Proc Natl Acad Sci USA 75:2035–2039Google Scholar
  29. Politoff AL, Blitz AL (1978) Neurotrophic control of RNA synthesis in amphibian striated muscle. Brain Res 151:561–570Google Scholar
  30. Popiela H (1978) Trophic effects of adult peripheral nerve extract on muscle cell growth and differentiation in vitro. Exp Neurol 62:405–416Google Scholar
  31. Prusik Z (1974) Free-flow electromigration separations. J Chromatogr 91:867–872Google Scholar
  32. Spencer PS (1972) Reapraisal of the model for “bulk” axoplasmic flow. Nature 240:283–285Google Scholar
  33. Tied TN, Albuquerque EX, Guth L (1977) Degenerating nerve fiber product do not alter physiological properties of adjanced innervated skeletal muscle fibers. Science 198:839–840Google Scholar
  34. Vrbová G (1967) Induction of an extrajunctional chemosensitive area in intact innervated muscle fibres. J Physiol 191:20–21PGoogle Scholar
  35. Vrbová, G (1970) Control of chemosensitivity at the neuromuscular junction. In: Eigenmann R (ed) Proc 4th int Congr Pharmacol, III. Swabe and Co, Basel, pp 158–169Google Scholar
  36. Vyskočil F, Syrový I (1979) Do peripheral nerves contain a factor inducing acetylcholine sensitivity in skeletal muscle? Experientia 35:218–219Google Scholar
  37. Younkin SG, Brett RS, Davey B, Younkin LH (1978) Substances moved by axonal transport and released by nerve stimulation have an innervation-like effect on the muscle. Science 200:1292–1295Google Scholar
  38. Weiss PA, Hiscoe HB (1948) Experiments in the mechanism of nerve growth. Exp Zool 107:315–395Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • F. Vyskočil
    • 1
  • I. Syrový
    • 1
  • Z. Prusík
    • 1
  1. 1.Institute of PhysiologyCzechoslovak Academy of SciencesPrague 4Czechoslovakia

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