, Volume 91, Issue 4, pp 554–560

Induced defence in detached uninfested plant leaves: effects on behaviour of herbivores and their predators

  • Marcel Dicke
  • Herman Dijkman
Original Papers


Induction of plant defence against herbivores may include the attraction by volatile infochemicals of natural enemies of the herbivore. The emitted volatiles that mediate this attraction may also affect the behaviour of the herbivore itself. In this paper we investigate the response of the herbivorous spider miteTetranychus urticae and the predatory mitePhytoseiulus persimilis towards volatiles whose production is induced in detached Lima bean leaves. Detached uninfested Lima bean leaves were incubated on wet cotton wool on which bean leaves infested with spider mites (T. urticae) were present simultaneously or had been present previously. These treatments induce the production of volatile infochemicals in the uninfested bean leaf tissue: predatory mites are attracted and spider mites are deterred. These are the first data on the response of predators and herbivores to plant volatiles whose production was induced in detached uninfested leaves.

Key words

Tritrophic interactions Lima bean Tetranychidae Phytoseiidae Herbivore-induced synomone 


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  1. Bowles D (1990) Signals in the wounded plant. Nature 343:314–315CrossRefGoogle Scholar
  2. Bruin J, Dicke M, Sabelis MW (1992) Plants are better protected against spider mites after exposure to volatiles from infested conspecifics. Experientia 48:525–529CrossRefGoogle Scholar
  3. Dicke M (1986) Volatile spider-mite pheromone and host-plant kairomone, involved in spaced-out gregariousness in the spider miteTetranychus urticae. Physiol Entomol 11:251–262Google Scholar
  4. Dicke M (1988) Prey preference of the phytoseiid miteTyphlodromus pyri: 1. Response to volatile kairomones. Exp Appl Acarol 4:1–13Google Scholar
  5. Dicke M, Sabelis MW (1988a) How plants obtain predatory mites as bodyguards. Neth J Zool 38:148–165Google Scholar
  6. Dicke M, Sabelis MW (1988b) Infochemical terminology: based on cost-benefit analysis rather than origin of compounds? Funct Ecol 2:131–139Google Scholar
  7. Dicke M, Sabelis MW (1989) Does it pay plants to advertize for bodyguards? Towards a cost-benefit analysis of induced synomone production. In: Lambers H, Cambridge ML, Konings H, Pons TL (eds), Causes & Consequences of Variation in Growth Rate and Productivity of Higher Plants SPB Academic Publishing, The Hague, pp 341–358Google Scholar
  8. Dicke M, Takabayashi J (1991) Specificity of induced indirect defence of plants against herbivores. Redia 74(3) [Suppl]:105–113Google Scholar
  9. Dicke M, Beek TA van, Posthumus MA, Ben Dom N, Bokhoven H van, Groot Æ de (1990a) Isolation and identification of volatile kairomone that affects acarine predator-prey interactions. Involvement of host plant in its production. J Chem Ecol 16:381–396Google Scholar
  10. Dicke M, Sabelis MW, Takabayashi J, Bruin J, Posthumus MA (1990b) Plant strategies of manipulating predator-prey interactions through allelochemicals: prospects for application in pest control. J Chem Ecol 16:3091–3118Google Scholar
  11. Dicke M, Maas KJ van der, Takabayashi J, Vet LEM (1990c) Learning affects response to volatile allelochemicals by predatory mites. Proc Exp Appl Entomol, N.E.V. (Amsterdam) 1:31–36Google Scholar
  12. Harrison S, Karban R (1986) Behavioural response of spider mites (Tetranychus urticae) to induced resistance of cotton plants? Ecol Entomol 11:181–188Google Scholar
  13. Haukioja E (1990) Induction of defenses in trees. Annu Rev Entomol 36:25–42Google Scholar
  14. Karban R, Carey JR (1984) Induced resistance of cotton seedlings to mites. Science 225: 53–54Google Scholar
  15. Karban R, Myers JH (1989) Induced plant responses to herbivory. Annu Rev Ecol Syst 20:331–348CrossRefGoogle Scholar
  16. Nordlund DA, Jones RL, Lewis WJ (eds) (1981) Semiochemicals, their role in pest control. Wiley & Sons, New YorkGoogle Scholar
  17. Pearce G, Strydom D, Johnson S, Ryan CA (1991) A polypeptide from tomato leaves induces wound-inducible proteinase inhibitor proteins. Science 253:895–898Google Scholar
  18. Price PW (1981) Semiochemicals in evolutionary time. In: Nordlund DA, Jones RL, Lewis WJ (eds), Semiochemicals: their use in pest control. Wiley, New York, pp 251–271Google Scholar
  19. Ryan CA, Farmer EE (1991) Oligosaccharide signals in plants: a current assessment. Annu Rev Plant Physiol Mol Biol 42:651–674CrossRefGoogle Scholar
  20. Sabelis MW, Van de Baan HE (1983) Location of distant spider mite colonies by phytoseiid predators: demonstration of specific kairomones emitted byTetranychus urticae andPanonychus ulmi. Entomol Exp Appl 33:303–314Google Scholar
  21. Takabayashi J, Dicke M (1992) Response of predatory mites with different rearing histories to volatiles of uninfested plants. Entomol Exp Appl (in press)Google Scholar
  22. Takabayashi J, Dicke M, Posthumus MA (1991a) Induction of indirect defence against spider-mites in uninfested Lima bean leaves. Phytochemistry 30:1459–1462CrossRefGoogle Scholar
  23. Takabayashi J, Dicke M, Posthumus MA (1991b) Variation in composition of predator-attracting allelochemicals emitted by herbivore-infested plants: relative influence of plant and herbivore. Chemoecology 2:1–6CrossRefGoogle Scholar
  24. Tallamy DW, Raupp MJ (eds) (1991) Phytochemical Induction by Herbivores. J. Wiley and Sons, New YorkGoogle Scholar
  25. Tomczyk A, Kropczynska D (1985) Effects on the host plant. In: Helle W, Sabelis MW (eds) Spider Mites. Their Biology, Natural Enemies and Control. (World Crop Pests Vol. 1A) Elsevier, Amsterdam, pp 317–329Google Scholar
  26. Turlings TCJ, Tumlinson JH (1991) Do parasitoids use herbivore-induced plant chemical defenses to locate hosts? Fla Entomol 74:42–50Google Scholar
  27. Turlings TCJ, Scheepmaker JWA, Vet LEM, Tumlinson JH, Lewis WJ (1990a) How contact foraging experiences affect preferences for host-related odors in the larval parasitoidCotesia marginiventris (Cresson) (Hymenoptera: Braconidae). J Chem Ecol 16:1577–1589CrossRefGoogle Scholar
  28. Turlings TCJ, Tumlinson JH, Lewis WJ (1990b) Exploitation of herbivore-induced plant odors by host-seeking parasitic wasps. Science 250:1251–1253Google Scholar
  29. Turlings TCJ, Tumlinson JH, Eller FJ, Lewis WJ (1991) Larval-damaged plants: source of volatile synomones that guide the parasitoidCotesia marginiventris to the micro-habitat of its hosts. Entomol Exp Appl 58:75–82CrossRefGoogle Scholar
  30. Vet LEM, Dicke M (1992) The ecology of infochemical use by natural enemies of herbivores in a tritrophic context. Annu Rev Entomol 37:141–172CrossRefGoogle Scholar
  31. Vinson SB (1976) Host selection by insect parasitoids. Annu Rev Entomol 21: 109–134CrossRefGoogle Scholar
  32. Whitman DW, Eller FJ (1990) Parasitic wasps orient to green leaf volatiles. Chemoecology 1:69–75CrossRefGoogle Scholar
  33. Zeringue HJ Jr (1987) Changes in cotton leaf chemistry induced by volatile elicitors. Phytochemistry 26:1357–1360Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Marcel Dicke
    • 1
  • Herman Dijkman
    • 1
  1. 1.Department of EntomologyWageningen Agricultural UniversityWageningenThe Netherlands

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