Histochemistry

, Volume 63, Issue 2, pp 245–251 | Cite as

Quantitative distribution of lysosomal hydrolases in the rat nephron

  • M. Le Hir
  • U. C. Dubach
  • U. Schmidt
Article
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Summary

The activities of N-acetyl-β,d-glucosaminidase (NAG, EC 3.2.1.30),β,d-galactosidase (β-gal, EC 3.2.1.23) and acid phosphatase (ac-Pase, EC 3.1.3.2) were measured in the glomeruli, five segments of the proximal and four segments of the distal tubule of normal male Wistar rats. The activities of NAG andβ-gal are 3- to 5-fold higher in the first part of the proximal tubule than in other segments and very low in glomeruli. We propose that the distribution of these two glycosidases reflects the contribution of the different tubular segments to the reabsorption of glycoproteins. The maximal activity of ac-Pase was found in the straight part of the proximal tubule. It was only 1.5-fold higher than in the distal tubule. Moreover, the activity in glomeruli is rather high. We conclude that ac-Pase is not primarily involved in the handling of reabsorbed molecules.

Keywords

Public Health Acid Phosphatase Maximal Activity Proximal Tubule Distal Tubule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • M. Le Hir
    • 1
  • U. C. Dubach
    • 1
  • U. Schmidt
    • 1
  1. 1.Medizinische Universitäts-Poliklinik, Departement ForschungKantonsspital BaselBaselSwitzerland

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