Lactate threshold and onset of blood lactate accumulation during incremental exercise after dietary modifications

  • A. Quirion
  • G. R. Brisson
  • L. Laurencelle
  • D. DeCarufel
  • A. Audet
  • S. Dulac
  • M. Ledoux
  • P. Vogelaere


This study was designed to clarify the effects of dietary modifications on the lactate threshold (LT) and on the onset of blood lactate accumulation (OBLA) during progressive incremental exercise. Six healthy males volunteered for the study. Informed consent was obtained from every participant. The following protocol was administered to each subject on three occasions: a 48-h period of mixed dieting (53% carbohydrates, 30% lipids, 17% proteins) preceding the first exercise test, immediately followed by a 48-h period of either a carbohydrate-rich (68% CHO, 23% lipids, 9% proteins) or a fat-rich (19% CHO, 57% lipids, 26% proteins) iso-caloric diet leading to the second exercise and separated from the third test by a 12-days period. Exercise tests were conducted on an electrically-braked ergocycle, and consisted of a progressive incremental maximal exercise. Respiratory parameters were continuously monitored by an automated open circuit sampling system. Exercise blood lactate (LA), free fatty acids (FFA), glucose levels and acid-base balance were determined from venous blood samples obtained through an indwelling brachial catheter. Peak lactate values, workload and performance time were not significantly altered by imposed diets. Furthermore, dietary modifications had no significant effect on LT, OBLA fixed at 4 mmol and ventilatory threshold. Increased pH and FFA mobilization were observed with fat-rich diet, while CHO-rich diet markedly increased the respiratory exchange ratio (R). It is concluded that LT and OBLA are not significantly altered by fat or CHO enrichment of diets.


Lactate threshold Onset on blood lactate accumulation Dietary modifications 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bergström J, Hermansen L, Hultman E (1967) Diet muscle glycogen and physical performance. Acta Physiol Scand 71:140–150Google Scholar
  2. Brozek J, Keys A (1951) The evaluation of leanness-fatness in man: Norms and interrelations. Br J Nutr 5:194–206Google Scholar
  3. Brozek J, Grande F, Anderson JT, Keys A (1963) Densitometric analysis of body composition: Revision of some quantitative assumptions. Ann NY Acad Sci 110:113–140Google Scholar
  4. Costill DL, Thompson H, Roberts E (1973) Fractional utilization of the aerobic capacity during distance running. Med Sci Sports 5:248–252Google Scholar
  5. Davis JA, Vodak P, Wilmore JH, Vodak J, Kurtz P (1976) Anaerobic threshold and maximal aerobic power for three modes of exercise. J Appl Physiol 41:544–550Google Scholar
  6. Farrel PA, Wilmore JH, Coyle EF, Billing JE, Costill DL (1979) Plasma lactate accumulation and distance running performance. Med Sci Sports Exerc 11:338–344Google Scholar
  7. Gollnick PD, Bagly WM, Hodgson DR (1986) Exercise intensity, training, diet, and lactate concentration in muscle and blood. Med Sci Sports Exerc 18(3):334–340Google Scholar
  8. Gutmann I, Wahlefeld AW (1974) In: Berganeyer HU: Methoden der enzymatischen Analyse, 3, Edizione, vol 11. Verlag Chemie, Weinheim, p 1510Google Scholar
  9. Henritze J, Weltman A, Schurrer RL, Barlow K (1985) Effects of training at and above the lactate threshold on the lactate threshold and maximal oxygen uptake. Eur J Appl Physiol 54:84–88Google Scholar
  10. Hughes EF, Turner SC, Brooks JA (1982) Effect of glycogen depletion and pedalling speed on “anaerobic threshold”. J Appl Physiol 52:1598–1607Google Scholar
  11. Hughson RL, Kowalchuk JM (1981) Influence of diet on CO2 production and ventilation in constant-load exercice. Respir Physiol 46:149–160Google Scholar
  12. Hultman E (1971) Muscle glycogen stores and prolonged exercise. In: Shephard RJ (ed) Frontiers of fitness. C C Thomas, Springfield, pp 37–60Google Scholar
  13. Ivy JL, Costill DL, Van Handel PJ, Essig DA, Lower RW (1981) Alteration in the lactate threshold with changes in substrate availability. Int J Sports Med 2:139–142Google Scholar
  14. Jacobs I (1981) Lactate, muscle glycogen and exercise performance in man. Acta Physiol Scand [Supp] 495Google Scholar
  15. Jacobs I, Kaiser P (1982) Lactate in blood mixed skeletal muscle and Ft or St fibers during cycle exercise in man. Acta Physiol Scand 114:461–466Google Scholar
  16. Jacobs I, Sjödin B, Kaiser P, Karlsson J (1981) Onset of blood lactate accumulation after prolonged exercise. Acta Physiol Scand 112:215–217Google Scholar
  17. Jansson E, Kaijser L (1982) Effect of diet on muscle glycogen and blood glucose utilization during a short-term exercise in man. Acta Physiol Scand 115:341–347Google Scholar
  18. Jones NL, Sutton JR, Taylor R, Toews CJ (1977) Effect of pH on cardiorespiratory and metabolic responses to exercise. J Appl Physiol 43:959–964Google Scholar
  19. Kelman GR, Maughan RJ, Williams C (1975) The effect of dietary modifications on blood lactate during exercise. J Physiol 251:34–35Google Scholar
  20. Kindermann W, Simon G, Keul J (1979) The significance of the aerobic-anaerobic transition for the determination of workload intensities during endurance training. Eur J Appl Physiol 42:25–34Google Scholar
  21. Kowalchuk JN (1980) The effect of dietary alterations on respiratory gas exchange during exercise. MSc Thesis University of WaterlooGoogle Scholar
  22. Kowalchuk JM, Heigenhauser GJF, Jones NL (1984) Effect of pH on metabolic and cardiorespiratory responses during progressive exercise. J Appl Physiol 57:1558–1563Google Scholar
  23. Mader A, Liesen H, Heck H, Philippi H, Rost R, Schurch P, Hollmann W (1976) Zur Beurteilung der sportartspezifischen Ausdauerleistungsfähigkeit im Labor. Sportarzt Sportmed 27:80–88Google Scholar
  24. Mader A, Hollman W (1977) Zur Bedeutung der Stoffwechselleitungsfähigkeit des Eliteruderers im Training und Wettkampf. Leistungssport 9:8–62Google Scholar
  25. Maughan RJ, Poole DC (1981) The effects of a glycogen-loading regimen on the capacity to perform anaerobic exercise. Eur J Appl Physiol 46:211–219Google Scholar
  26. Maughan RJ, Williams C, Campbell DM, Hepburn D (1978) Fat and carbohydrate metabolism during low intensity exercise: Effects of the availability of muscle glycogen. Eur J Appl Physiol 39:7–16Google Scholar
  27. Neary PJ, MacDougall JD, Bachus R, Wenger HA (1985) The relationship between lactate and ventilatory thresholds: coincidental or cause and effect? Eur J Appl Physiol 54:104–108Google Scholar
  28. Noma A, Okabe H, Kita M (1973) A new colorimetric microdetermination of free fatty acids in serum. Clin Chim Acta 43:317–320Google Scholar
  29. Pascale LR, Grossman MJ, Sloan HS, Frankel T (1956) Correlations between thickness of skinfolds and body density in 88 soldiers. Human Biol 28:165–176Google Scholar
  30. Pinelli A (1973) A new colorimetric method for plasma free fatty acid analysis. Clin Chim Acta 44:385–390Google Scholar
  31. Rennie MS, Johnson RH (1974) Effects of an exercise-diet program on metabolic changes with exercise in runners. J Appl Physiol 37:821–825Google Scholar
  32. Saltin B, Hermansen L (1967) Glycogen stores and prolonged severe exercise. In: Blix (ed) Symposia of the Swedish Nutrition Foundation. 5. Nutrition and physical activity. Almquist, Wiksell, Uppsala, pp 32–46Google Scholar
  33. Siggard-Andersen O (1974) The acid-base status of blood. 4th Revised edition. Munksgaard, CopenhagenGoogle Scholar
  34. Sjödin B, Jacobs I (1981) Onset of blood lactate accumulation and marathon running performance. Int J Sports Med 2:23–26Google Scholar
  35. Sloan AW (1967) Estimation of body fat in young men. J Appl Physiol 23:311–315Google Scholar
  36. Sutton JR, Jones NL, Toews CJ (1981) Effect of pH on muscle glycolysis during exercise. Clin Sci 61:331–338Google Scholar
  37. Wasserman K (1967) Lactate and related acid base and blood gas changes during constant load and graded exercise. Can Med Ass J 96:775–779Google Scholar
  38. Weltman A, Katch VC (1979) Relationship between the onset of metabolic acidosis (anaerobic threshold) and maximal oxygen uptake. J Sports Med 19:133–142Google Scholar
  39. Winer RJ (1971) Statistical principles in experimental design. McGraw Hill, New-YorkGoogle Scholar
  40. Yoshida T (1984) Effect of dietary modifications on lactate threshold and onset of blood lactate accumulation during incremental exercise. Eur J Appl Physiol 53:200–205Google Scholar
  41. Yoshida T (1986) Effect of dietary modifications on anaerobic threshold. Sports Med 3:4–9Google Scholar
  42. Yoshida T, Nagata A, Muro M, Takeuchi N, Suda Y (1981) The validity of anaerobic threshold determination by a Douglas bag method compared with arterial blood lactate concentration. Eur J Appl Physiol 46:423–430Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • A. Quirion
    • 1
  • G. R. Brisson
    • 2
  • L. Laurencelle
    • 1
  • D. DeCarufel
    • 1
  • A. Audet
    • 4
  • S. Dulac
    • 1
  • M. Ledoux
    • 3
  • P. Vogelaere
    • 5
  1. 1.Département des sciences de l'activité physiqueUniversité du Québec à Trois-RivièresTrois-Rivières
  2. 2.INRS-SANTÉUniversité du QuébecMontréal
  3. 3.Université de MontréalMontréal
  4. 4.Centre Hospitalier St-JosephTrois-RivièresCanada
  5. 5.Vrije Universiteit BrusselsBelgium

Personalised recommendations