Construction of plasmid-free derivatives ofSalmonella typhimurium LT2 using temperature-sensitive mutants of pKZ1 for displacement of the resident plasmid, pSLT
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Summary
Replication (or partitioning) temperature-sensitive mutants of pKZ1 were isolated and shown to exhibit incompatibility with the resident plasmid (pSLT) ofSalmonella typhimurium LT2. Following displacement of pSLT, the mutant plasmids were effectively eliminated from the cell population by passage at 42° C, yielding plasmid-free isolates.
Key words
Plasmid incompatibility pSLT pKZ1 Temperature-sensitive mutantsPreview
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References
- Anderson ES, Pitton JS, Mayhew JN (1968) Restriction of bacteriophage multiplication by resistance determinants in Salmonellae. Nature 219:640–641Google Scholar
- Bergquist PL (1987) Incompatibility. In: Hardy KG (ed) Plasmidsa practical approach. IRL Press, Oxford, pp 37–78Google Scholar
- Cerin H, Hackett J (1989) Molecular cloning and analysis of the incompatibility and partition functions of the virulence plasmid ofSalmonella typhimurium. Microbiol Pathol 7:85–99Google Scholar
- Gulig PA, Curtiss III R (1987) Plasmid-associated virulence ofSalmonella typhimurium. Infect Immun 55:2891–2901Google Scholar
- Hashimoto T, Sekiguchi M (1976) Temperature-sensitive mutants of R plasmid by in vitro mutagenesis with hydroxylamine. J Bacteriol 127:1561–1563Google Scholar
- Kelln RA, Kinahan JJ, Foltermann KF, O'Donovan GA (1975) Pyrimidine biosynthetic enzymes ofSalmonella typhimurium, repressed specifically by growth in the presence of cytidine. J Bacteriol 124:764–774Google Scholar
- Kleckner N, Chan RK, Type B-K, Botstein D (1975) Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. J Mol Biol 97:561–575Google Scholar
- Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New YorkGoogle Scholar
- Michiels T, Popoff MY, Durviaux S, Coynault C, Cornelis G (1987) A new method for the physical and genetic mapping of large plasmids: application to the localization of the virulence determinants on the 90 kb plasmid ofSalmonella typhimurium. Microbiol Pathol 3:109–116Google Scholar
- Minton NP (1984) Improved plasmid vectors for the isolation of translational lac gene fusions. Gene 31:269–273Google Scholar
- Neuhard J, Stauning E, Kelln RA (1985) Cloning and characterization of thepyrE gene ofpyrE::Mud1 (ApR lac) fusions fromSalmonella typhimurium. Eur J Biochem 146:597–603Google Scholar
- Novick RP (1987) Plasmid incompatibility. Microbiol Rev 51:381–395Google Scholar
- Roth JR (1970) Genetic techniques in studies of bacterial metabolism. Methods Enzymol 27A:3–35Google Scholar
- Sanderson KE, Kadam SK, MacLachlan PR (1983) Derepression of F factor function inSalmonella typhimurium. Can J Microbiol 29:1205–1212Google Scholar
- Smith HR, Humphreys GO, Grindley NDF, Grindley JN, Anderson ES (1973) Molecular studies of a fi+ plasmid from strains ofSalmonella typhimurium. Mol Gen Genet 126:143–151Google Scholar
- Vandenbosch JL, Rabert DK, Jones GW (1987) Plasmid associated resistance ofSalmonella typhimurium to complement activated by the classical pathway. Infect Immun 55:2645–2652Google Scholar
- Woodward MJ, McLaren I, Wray C (1989) Distribution of virulence plasmids within salmonellae. J Gen Microbiol 135:503–511Google Scholar
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