Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Structural variability in the genome of theThermoproteus tenax virus TTV1

  • 36 Accesses

  • 11 Citations


Six variants of the TTV1 genome, including the primary isolate, have been characterized. DNA sequence comparison of ‘wild-type’ virus (WT) and one of the variants (VT3) showed that differences are due to insertions and deletions that were confined to contiguous portions of two distinctClaI fragments. Seven similar short DNA sequences (30–102 bp) were involved in the variation. The deletions and insertions of these short DNA sequences occurred in every case adjacent to the 8 by consensus sequence 5′-ACXCCTAC-3′ which formed the 5′ flank of the segments involved.

This is a preview of subscription content, log in to check access.


  1. Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395

  2. Jankovic D, Wunderl S, Holz I, Zillig W, Gierl A, Neumann H (1983) TTV1, TTV2, and TTV3, a family of viruses of the extremely thermophilic, anaerobic, sulfur reducing archaebacteriumThermoproteus tenax. Mol Gen Genet 192:39–45

  3. Kleckner N (1981) Transposable elements in procaryotes. Annu Rev Genet 15:341–404

  4. Messing J, Vieira J (1982) A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene 19:269–276

  5. Neumann H, Zillig W (1990 a) The TTV1-encoded viral protein TPX: primary structure of the gene and the protein. Nucleic Acids Res 18:195

  6. Neumann H, Zillig W (1990b) Nucleotide sequence of the viral protein TPX of the TTV1 variant VT3. Nucleic Acids Res 18:2171

  7. Neumann H, Eckerskorn C, Schwass V, Zillig W (1989) Identification and characterization of the genes encoding three structural proteins of theThermoproteus tenax virus TTV 1. Mol Gen Genet 219:105–110

  8. Sanger F, Nicklen S, Coulsen AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

  9. Sanger F, Coulsen AR, Barrell BG, Smith AJH, Roe BA (1980) Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol 143:161–178

  10. Staden R (1980) A new computer method for the storage and manipulation of DNA gel reading data. Nucleic Acids Res 8:3673–3694

Download references

Author information

Correspondence to Wolfram Zillig.

Additional information

Communicated by J. Lengeler

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Neumann, H., Zillig, W. Structural variability in the genome of theThermoproteus tenax virus TTV1. Mol Gen Genet 222, 435–437 (1990).

Download citation

Key words

  • Thermoproteus
  • Virus
  • Variability