Molecular and General Genetics MGG

, Volume 222, Issue 2–3, pp 369–376 | Cite as

DNA sequence analysis of therecA genes fromProteus vulgaris, Erwinia carotovora, Shigella flexneri andEscherichia coli B/r

  • XiaoJun Zhao
  • Kevin McEntee
Article

Summary

The complete nucleotide sequences of therecA genes fromEscherichia coli B/r,Shigella flexneri, Erwinia carotovora andProteus vulgaris were determined. The DNA sequence of the coding region of theE. coli B/r gene contained a single nucleotide change compared with theE. coli K12 gene sequence whereas theS. flexneri gene differed at 7 residues. In both cases, the predicted proteins were identical in primary structure to theE. coli K12 RecA protein. The DNA sequences of the recA genes fromE. carotovora andP. vulgaris were 80% and 74% homologous, respectively, to theE. coli K12 gene. The predicted amino acid sequences of theE. carotovora andP. vulgaris RecA proteins were 91% and 85% identical respectively, to that ofE. coli K12. The RecA proteins from bothP. vulgaris andE. carotovora diverged significantly in sequence in the last 50 residues whereas they showed striking conservation throughout the first 300 amino acids which include an ATP-binding region and a subunit interaction domain. A putative LexA repressor binding site was localized upstream of each of the heterologous genes.

Key words

Conserved domains ATP binding LexA operator Carboxyl terminus 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • XiaoJun Zhao
    • 1
  • Kevin McEntee
    • 1
  1. 1.Department of Biological Chemistry, and Laboratory of Biomedical and Environmental Sciences, UCLA School of MedicineUniversity of CaliforniaLos AngelesUSA

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