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A convenient method for repeated intracellular recording of action potentials from the same muscle fibre without membrane damage

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Summary

Repeated intracellular recording of end-plate and action potentials from the same muscle fibre is often impossible because the membrane is damaged by the microelectrode when the muscle twitches. Membrane damage can be avoided by rolling and simultaneously stretching the muscle around a polyethylene rod; the amount of stretch necessary is about 50% of the “in-situ” length.

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References

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Additional information

Supported by the Deutsche Forschungsgemeinschaft, Sonderforschungsbereich 38 “Membranforschung”, Bad Godesberg.

Established Investigator of the Consejo Nacional de Investigaciones Científicas y Tecnicas, Argentina

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Stefani, E., Schmidt, H. A convenient method for repeated intracellular recording of action potentials from the same muscle fibre without membrane damage. Pflügers Arch. 334, 276–278 (1972). https://doi.org/10.1007/BF00626229

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Key words

  • Muscle Fibre
  • Intracellular Recording
  • Membrane Damage