Blut

, Volume 54, Issue 6, pp 351–359

CFU-F circulating in cord blood

  • G. Prindull
  • Z. Ben-Ishay
  • W. Ebell
  • M. Bergholz
  • T. Dirk
  • B. Prindull
Original Articles

Summary

CFU-F (colony forming units-fibroblast) were studied from cord blood and, as controls, from normal bone marrow of older children and adults. Numbers of CFU-F in cord blood buffy coat cells are lower by a factor of 10 in comparison to bone marrow CFU-F. Cytomorphology and staining with monoclonal antibody identify the progeny cells of CFU-F as fibroblasts. Cord blood CFU-F derived fibroblasts have properties supporting hematopoiesis: They produce CSF (colony stimulating factor) to which fresh cord blood CFU-GM (colony forming units-granulocytic, monocytic) react by colony formation in a dose-response manner. In addition, fibroblast colonies discharge clonogenic round cells into the medium forming CFU-GM and CFU-F colonies in secondary methyl cellulose cultures. We conclude that fetal blood contains clonogenic stromal cells (CFU-F) that give rise to fibroblasts with properties of hematopoietic support.

Key words

CFU-F Clonogenic Cord blood Fibroblasts 

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • G. Prindull
    • 1
  • Z. Ben-Ishay
    • 2
  • W. Ebell
    • 3
  • M. Bergholz
    • 4
  • T. Dirk
    • 4
  • B. Prindull
    • 1
  1. 1.Department of PediatricsUniversitäts-KinderklinikGöttingenGermany
  2. 2.Department of Anatomy and EmbryologyHadassah Medical SchoolJerusalemIsrael
  3. 3.Department of PediatricsUniversity of UlmUlmGermany
  4. 4.Department of PathologyUniversitäts-KinderklinikGöttingenGermany

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