Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Is the period of the circadian oscillator in the eye ofAplysia directly homeostatically regulated?

  • 19 Accesses

  • 26 Citations

Summary

The effect on the circadian oscillator in the isolatedAplysia eye of a number of agents which alter Ca2+ uptake or release by internal membranes has been investigated. The period of the oscillator is increased considerably by the methyl xanthines, Li+, Mn2+, and low concentrations of La3+. The effect of La3− on the oscillator can be reversed with butacaine which also inhibits the blocking effect La3+ has on Ca2+ uptake by mitochondria. The results of this study and other recent work lead us to conclude that the period of the circadian oscillator in the isolatedAplysia eye is most likely indirectly regulated.

This is a preview of subscription content, log in to check access.

Abbreviations

τ:

circadian oscillator period

ASW :

artificial sea water

8 BTcAMP :

8 benzyl-thio-cyclic adenosine monophosphate

8 PCPTcGMP :

8 parachlorophenyl-thio-cyclic guanosine monophosphate

CAP compound action potential:

CR circadian rhythm

IBMX :

3 isobutyl 1-methyl xanthine

SEM :

standard error of the mean

References

  1. Audesirk G (1973) Spontaneous and light-induced compound action potentials in the isolated eye ofAplysia: initiation and synchronization. Brain Res 59:229–242

  2. Baker PF, Hodgkin AL, Ridgeway EB (1971) Depolarization and calcium entry in squid giant axons. J Physiol (Lond) 218:709–755

  3. Benson JA, Jacklet JW (1977) Circadian rhythm of output from neurons in the eye ofAplysia, I: Effects of deuterium oxide and temperature. J Exp Biol 70:151–166

  4. Blayney L, Thomas H, Muir J, Henderson A (1978) Action of caffeine on calcium transport by isolated fractions of myofibrils, mitochondria, and sarcoplasmic reticulum from rabbit heart. Circ Res 43:520–526

  5. Bollig I, Mayer K, Mayer WE, Engelmann W (1978) Effects of cAMP, theophylline, imidazole, and 4-(3,4-dimethoxy-benzyl)-2-imidazolidone on the leaf movement rhythm ofTrifolium repens — a test of the cAMP hypothesis of circadian rhythms. Planta 141:225–230

  6. Brinley FJ Jr (1978) Calcium buffering in squid axons. Annu Rev Biophys Bioeng 7:363

  7. Constanti MG, Sturani EP, Ghersa P, Alberghina L (1978) Effects of caffeine on RNA and protein synthesis inNeurospora crassa. Exp Mycol 2:366–376

  8. Crawford AC (1975) Lithium ions and the release of transmitter at the frog neuromuscular junction. J Physiol (Lond) 246:109–142

  9. Daan S, Pittendrigh CS (1976) A functional analysis of circadian pacemakers in nocturnal rodents III. Heavy water and constant light: homeostasis of frequency? J Comp Physiol 106:267–290

  10. Engelmann W (1972) Lithium slows down theKalanchoe clock. Z Naturforsch B 27:477

  11. Eskin A (1977) Entraining a circadian rhythm from the isolated eye of Aplysia: the involvement of changes in membrane potential. Soc Neurosci Abstr 3:176

  12. Eskin A (1982) Differential effects of amino acids on the period of the circadian rhythm from theAplysia eye. J Neurobiol 13:231–239

  13. Eskin A, Corrent G (1977) Effects of divalent cations and metabolic poisons on the circadian rhythm from theAplysia eye. J Comp Physiol 117:1–21

  14. Feldman J (1975) Circadian periodicity inNeurospora: Alteration by inhibitors of cyclic AMP phosphodiesterase. Science 190:789–790

  15. Goodenough JE, Bruce VG (1980) The effects of caffeine and theophylline on the photoactic rhythm ofChlamydomonas reinhardii. Biol Bull 159:649–655

  16. Gwinner E (1974) Testerosterone induces “splitting” of circadian locomotor activity rhythms in birds. Science 185:72–74

  17. Hagiwara S, Byerly L (1981) Calcium channel. Annu Rev Neurosci 4:69–125

  18. Hagiwara S, Takahashi K (1967) Surface density of calcium ions and calcium spikes in the barnacle muscle fiber membrane. J Gen Physiol 50:583–601

  19. Kripke DF, Wyborney G (1980) Lithium slows rat circadian activity rhythms. Life Sci 26:1319–1321

  20. Kuba K (1980) Release of calcium ions linked to the activation of potassium conductance in a caffeine-treated sympathetic neurone. J Physiol (Lond) 298:251–269

  21. Mela L (1968) Interactions of La3+ and local anesthetic drugs with mitochondrial Ca2+ and Mn2+ uptake. Arch Biochem Biophys 123:286–293

  22. Morin LP, Fitzgerald KM, Zucker I (1977) Estradiol shortens the period of hamster circadian rhythms. Science 196:305–307

  23. Pittendrigh CS (1974) Circadian oscillations in cells and the circadian organization of multicellular systems. In: Schmitt FO, Worden FG (eds) The neurosciences. Third study program. The MIT Press, Cambridge, Massachusetts, pp 437–458

  24. Pittendrigh C, Daan S (1976) A functional analysis of circadian pacemakers in nocturnal rodents: I. The stability and lability of spontaneous frequency. J Comp Physiol 106:223–252

  25. Pittendrigh CS, Caldarola PC, Cosbey ES (1973) A differential effect of heavy water on temperature dependent and temperature-compensated aspects of the circadian system ofDrosophila pseudoobscura. Proc Natl Acad Sci USA 70:2037–2041

  26. Turek FW, McMillan JP, Menaker MI (1976) Melatonin: effects on the circadian locomotor rhythm of sparrows. Science 194:441–1443

  27. Weber A, Herz R (1968) The relationship between caffeine contracture of intact muscle and the effect of caffeine on the reticulum. J Gen Physiol 52:750–759

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Woolum, J.C., Strumwasser, F. Is the period of the circadian oscillator in the eye ofAplysia directly homeostatically regulated?. J. Comp. Physiol. 151, 253–259 (1983). https://doi.org/10.1007/BF00623902

Download citation

Keywords

  • Methyl
  • Recent Work
  • Xanthine
  • Blocking Effect
  • Methyl Xanthine