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Immunogenetics

, Volume 15, Issue 2, pp 151–166 | Cite as

Evolution of HLA antigenic determinants: Species cross-reactions of monoclonal antibodies

  • Frances M. Brodsky
  • Peter Parham
Original Investigations

Abstract

The reactions of mouse monoclonal HLA-A-, -B-, -C-specific antibodies on cells from 50 species were analyzed by binding assay. Antibodies which are monomorphic in humans generally show monomorphic behavior in other species and many significant cross-reactions were seen. Antibodies which recognize highly specific polymorphic determinants gave few cross-reactions with other species. Antibodies which recognize broad polymorphic determinants in humans cross-reacted in a polymorphic manner with many other species. The patterns of cross-reaction for both monomorphic and broadly polymorphic determinants correlated roughly with the current picture of phylogenetic relationships. These results show there are two fundamentally different classes of polymorphic determinants, one which appears relatively conserved in evolution and one which is the product of recent change. They probably correlate with the public and private antigens of mouse H-2 antigens. A simple genetic model ofHLA-A, -B, -C genes, whereby all specificities at a locus are true alleles, is sufficient to explain these patterns of cross-reaction. It seems likely that cross-reactions previously seen with alloantisera were due to broadly polymorphic antibodies rather than to allele-specific antibodies. If this were the case, then no experimental basis for Bodmer's model of MHC polymorphism being due to control of expression of a tandem array of genes exists.

Keywords

Monoclonal Antibody Phylogenetic Relationship Genetic Model Binding Assay Recent Change 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used in this paper

MHC

major histocompatibility complex

PBS

phosphate-buffered saline

BSA

bovine serum albumin

RAM

rabbit anti-mouse IgG F(ab′)2 fragments

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Frances M. Brodsky
    • 1
  • Peter Parham
    • 1
  1. 1.Department of Structural BiologyStanford University School of MedicineStanford

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