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Optical and Quantum Electronics

, Volume 24, Issue 8, pp 851–859 | Cite as

Application of a femtosecond self-sustaining mode-locked Ti:sapphire laser to the field of laser scanning confocal microscopy

  • P. F. Curley
  • A. I. Ferguson
  • J. G. White
  • W. B. Amos
Special Features

Abstract

Developments in ultrafast Ti:sapphire laser technology can be applied in the investigation of nonlinear optical processes. We describe the application of a self-sustaining femtosecond Ti:sapphire laser as an illumination source in the field of confocal laser scanning fluorescence microscopy (LSM). We present spectra for various fluorescent stains under two-photon excitation and present LSM images of stained samples under mode-locked illumination. The potential for such a system as a non-destructive technique for studying live cells in biomedical research is discussed.

Keywords

Live Cell Communication Network Confocal Microscopy Sapphire Laser Scanning Confocal Microscopy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman & Hall 1992

Authors and Affiliations

  • P. F. Curley
    • 1
  • A. I. Ferguson
    • 1
  • J. G. White
    • 2
  • W. B. Amos
    • 2
  1. 1.Department of Physics and Applied PhysicsUniversity of StrathclydeGlasgowUK
  2. 2.Laboratory of Molecular BiologyMedical Research CouncilCambridgeUK

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